Construct: ORF TRCN0000476141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017545.1_s317c1
- Derived from:
- ccsbBroadEn_10618
- DNA Barcode:
- GCCGAACCGGTTGTTCTTGATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LINC00965 (349196)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476141
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 349196 | LINC00965 | long intergenic non-protein... | NR_027000.1 | 9.3% | 1_1502del;1773_2874del | |
| 2 | human | 112268400 | LOC112268400 | uncharacterized LOC112268400 | XR_002959170.1 | 7.2% | 1_1635del;1695G>A;1906_3716del | |
| 3 | human | 112268389 | LOC112268389 | uncharacterized LOC112268389 | XR_002959145.1 | 7% | (many diffs) | |
| 4 | human | 112268389 | LOC112268389 | uncharacterized LOC112268389 | XR_002959149.1 | 6.9% | (many diffs) | |
| 5 | human | 112268389 | LOC112268389 | uncharacterized LOC112268389 | XR_002959143.1 | 6.7% | (many diffs) | |
| 6 | human | 112268400 | LOC112268400 | uncharacterized LOC112268400 | XR_002959169.1 | 6.7% | 1_1915del;1975G>A;2186_3996del | |
| 7 | human | 112268389 | LOC112268389 | uncharacterized LOC112268389 | XR_002959148.1 | 6.6% | (many diffs) | |
| 8 | human | 112268400 | LOC112268400 | uncharacterized LOC112268400 | XR_002959167.1 | 6.4% | 1_2086del;2146G>A;2357_4167del | |
| 9 | human | 112268389 | LOC112268389 | uncharacterized LOC112268389 | XR_002959140.1 | 6.4% | (many diffs) | |
| 10 | human | 100128890 | FAM66B | family with sequence simila... | NR_027423.2 | 6% | (many diffs) | |
| 11 | human | 112268400 | LOC112268400 | uncharacterized LOC112268400 | XR_002959168.1 | 5.6% | 1_2688del;2748G>A;2959_4769del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 339
- ORF length:
- 270
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGTGTCAGAC ATCCGGAAAG AAGACGGGAT GAATGTTTTA CCTCTGAAGT 121 ACATCCCAAA TGTGGGAGTT AACTTCAGCT TTGCTGGGGT CTATTTGGCC AGTGAAACTC 181 TGCCTGGTTC CTTCGCACAT CCGGAAGCCA CTTCACGGGG GGCCGTCGCA ACTGGAACCA 241 CACACTTGGC ATCGGCGGTT GAGCCAAATG GGGACTCGTG GTGCAAGCAA CGCTCCCCAC 301 GTGTTAGCGT GCGTGAGATG CGGTTGGCGG AATTTTACTT GCCAACTTTC TTGTACAAAG 361 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 421 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 481 ACGAGCCGAA CCGGTTGTTC TTGATCTCAC GCGTTAAGTC gacaatcaac ctctggatta 541 caaaatttgt gaaagatt