Construct: ORF TRCN0000476170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017285.1_s317c1
- Derived from:
- ccsbBroadEn_03475
- DNA Barcode:
- CCAAAGGCGACAAGCGGTCCTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF43 (54894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54894 | RNF43 | ring finger protein 43 | NM_001305544.2 | 100% | 100% | |
| 2 | human | 54894 | RNF43 | ring finger protein 43 | NM_017763.5 | 100% | 100% | |
| 3 | human | 54894 | RNF43 | ring finger protein 43 | XM_011524955.3 | 89.7% | 88.2% | (many diffs) |
| 4 | human | 54894 | RNF43 | ring finger protein 43 | XM_017024800.2 | 89.7% | 88.2% | (many diffs) |
| 5 | human | 54894 | RNF43 | ring finger protein 43 | NM_001305545.1 | 83.7% | 83.7% | 0_1ins381 |
| 6 | human | 54894 | RNF43 | ring finger protein 43 | XM_011524956.3 | 75.1% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2418
- ORF length:
- 2349
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtggtggc caccagctgc agctggctgc cctctggccc tggctgctga 121 tggctaccct gcaggcaggc tttggacgca caggactggt actggcagca gcggtggagt 181 ctgaaagatc agcagaacag aaagctatta tcagagtgat ccccttgaaa atggacccca 241 caggaaaact gaatctcact ttggaaggtg tgtttgctgg tgttgctgaa ataactccag 301 cagaaggaaa attaatgcag tcccacccgc tgtacctgtg caatgccagt gatgacgaca 361 atctggagcc tggattcatc agcatcgtca agctggagag tcctcgacgg gccccccgcc 421 cctgcctgtc actggctagc aaggctcgga tggcgggtga gcgaggagcc agtgctgtcc 481 tctttgacat cactgaggat cgagctgctg ctgagcagct gcagcagccg ctggggctga 541 cctggccagt ggtgttgatc tggggtaatg acgctgagaa gctgatggag tttgtgtaca 601 agaaccaaaa ggcccatgtg aggattgagc tgaaggagcc cccggcctgg ccagattatg 661 atgtgtggat cctaatgaca gtggtgggca ccatctttgt gatcatcctg gcttcggtgc 721 tgcgcatccg gtgccgcccc cgccacagca ggccggatcc gcttcagcag agaacagcct 781 gggccatcag ccagctggcc accaggaggt accaggccag ctgcaggcag gcccggggtg 841 agtggccaga ctcagggagc agctgcagct cagcccctgt gtgtgccatc tgtctggagg 901 agttctctga ggggcaggag ctacgggtca tttcctgcct ccatgagttc catcgtaact 961 gtgtggaccc ctggttacat cagcatcgga cttgccccct ctgcatgttc aacatcacag 1021 agggagattc attttcccag tccctgggac cctctcgatc ttaccaagaa ccaggtcgaa 1081 gactccacct cattcgccag catcccggcc atgcccacta ccacctccct gctgcctacc 1141 tgttgggccc ttcccggagt gcagtggctc ggcccccacg acctggtccc ttcctgccat 1201 cccaggagcc aggcatgggc cctcggcatc accgcttccc cagagctgca catccccggg 1261 ctccaggaga gcagcagcgc ctggcaggag cccagcaccc ctatgcacaa ggctggggac 1321 tgagccacct ccaatccacc tcacagcacc ctgctgcttg cccagtgccc ctacgccggg 1381 ccaggccccc tgacagcagt ggatctggag aaagctattg cacagaacgc agtgggtacc 1441 tggcagatgg gccagccagt gactccagct cagggccctg tcatggctct tccagtgact 1501 ctgtggtcaa ctgcacggac atcagcctac agggggtcca tggcagcagt tctactttct 1561 gcagctccct aagcagtgac tttgaccccc tagtgtactg cagccctaaa ggggatcccc 1621 agcgagtgga catgcagcct agtgtgacct ctcggcctcg ttccttggac tcggtggtgc 1681 ccacagggga aacccaggtt tccagccatg tccactacca ccgccaccgg caccaccact 1741 acaaaaagcg gttccagtgg catggcagga agcctggccc agaaaccgga gtcccccagt 1801 ccaggcctcc tattcctcgg acacagcccc agccagagcc accttctcct gatcagcaag 1861 tcaccagatc caactcagca gccccttcgg ggcggctctc taacccacag tgccccaggg 1921 ccctccctga gccagcccct ggcccagttg acgcctccag catctgcccc agtaccagca 1981 gtctgttcaa cttgcaaaaa tccagcctct ctgcccgaca cccacagagg aaaaggcggg 2041 ggggtccctc cgagcccacc cctggctctc ggccccagga tgcaactgtg cacccagctt 2101 gccagatttt tccccattac acccccagtg tggcatatcc ttggtcccca gaggcacacc 2161 ccttgatctg tggacctcca ggcctggaca agaggctgct accagaaacc ccaggcccct 2221 gttactcaaa ttcacagcca gtgtggttgt gcctgactcc tcgccagccc ctggaaccac 2281 atccacctgg ggaggggcct tctgaatgga gttctgacac cgcagagggc aggccatgcc 2341 cttatccgca cTGCCAGGTG CTGTCGGCCC AGCCTGGCTC AGAGGAGGAA CTCGAGGAGC 2401 TGTGTGAACA GGCTGTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2461 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2521 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCAAAGG CGACAAGCGG 2581 TCCTCACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt