Transcript: Human XM_011524955.3

PREDICTED: Homo sapiens ring finger protein 43 (RNF43), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RNF43 (54894)
Length:
3555
CDS:
901..3510

Additional Resources:

NCBI RefSeq record:
XM_011524955.3
NBCI Gene record:
RNF43 (54894)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011524955.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004217 CCATGTGAGGATTGAGCTGAA pLKO.1 1446 CDS 100% 4.050 5.670 N RNF43 n/a
2 TRCN0000422519 ATCACAGAGGGAGATTCATTT pLKO_005 1846 CDS 100% 13.200 9.240 N RNF43 n/a
3 TRCN0000424439 GCAGAACAGAAAGCTATTATC pLKO_005 1024 CDS 100% 13.200 9.240 N RNF43 n/a
4 TRCN0000422812 ATGCCAGTGATGACGACAATC pLKO_005 1175 CDS 100% 10.800 7.560 N RNF43 n/a
5 TRCN0000422557 TAGTGTACTGCAGCCCTAAAG pLKO_005 2423 CDS 100% 10.800 7.560 N RNF43 n/a
6 TRCN0000004218 CCACCTCCAATCCACCTCACA pLKO.1 2157 CDS 100% 0.880 0.616 N RNF43 n/a
7 TRCN0000010861 CTCCACCTCATTCGCCAGCAT pLKO.1 1915 CDS 100% 0.880 0.616 N RNF43 n/a
8 TRCN0000004216 GAAGGTGTGTTTGCTGGTGTT pLKO.1 1096 CDS 100% 4.050 2.430 N RNF43 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011524955.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03475 pDONR223 100% 89.7% 88.2% None (many diffs) n/a
2 ccsbBroad304_03475 pLX_304 0% 89.7% 88.2% V5 (many diffs) n/a
3 TRCN0000476170 CCAAAGGCGACAAGCGGTCCTCAC pLX_317 3.2% 89.7% 88.2% V5 (many diffs) n/a
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