Construct: ORF TRCN0000476172
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008628.1_s317c1
- Derived from:
- ccsbBroadEn_08358
- DNA Barcode:
- CACAGGGTCAACGAGCAAGTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPSF2 (53981)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476172
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53981 | CPSF2 | cleavage and polyadenylatio... | NM_001322272.2 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 53981 | CPSF2 | cleavage and polyadenylatio... | NM_017437.3 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 53981 | CPSF2 | cleavage and polyadenylatio... | NM_001322270.2 | 93.3% | 93.2% | (many diffs) |
| 4 | human | 53981 | CPSF2 | cleavage and polyadenylatio... | NM_001322271.2 | 80.2% | 80.1% | (many diffs) |
| 5 | mouse | 51786 | Cpsf2 | cleavage and polyadenylatio... | NM_016856.3 | 88.5% | 97.6% | (many diffs) |
| 6 | mouse | 51786 | Cpsf2 | cleavage and polyadenylatio... | XM_006516071.3 | 88.5% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2415
- ORF length:
- 2346
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacgtctatt atcaaattaa ctaccctttc tggggtccaa gaagaatctg 121 ccctttgcta tcttctccaa gttgatgagt ttagattttt attggactgt ggctgggatg 181 agcacttttc tatggatatt attgattccc tgaggaagca tgttcaccag attgatgcag 241 tgctgttgtc tcaccctgat cctctccacc ttggtgccct cccgtatgct gtcggaaagt 301 tgggtctgaa ctgtgctatc tatgcaacca ttcctgttta taaaatggga cagatgttca 361 tgtatgatct ttatcagtct cgacacaata cagaagattt tacactcttt acattagatg 421 atgtggatgc agcctttgat aaaatacagc agctaaaatt ctctcagatt gtgaatttga 481 aaggtaaagg acatggcctg tctatcacac ctctgccagc tggtcatatg ataggtggaa 541 caatatggaa aatagtcaaa gatggagaag aagaaattgt ttatgcagtt gacttcaacc 601 acaagaggga gatccattta aatggatgtt ccctggaaat gctaagcagg ccttccctac 661 ttatcacaga ttcattcaat gctacatatg tacagcctag aagaaaacag agagatgagc 721 agcttctgac aaatgtcctg gaaacacttc gaggtgatgg aaatgtgtta atagcagtgg 781 acacagcagg cagagttttg gaacttgctc aacttcttga tcagatttgg aggactaaag 841 atgcaggatt gggtgtttac tcattggcac tcctaaataa tgtcagttac aatgtggtgg 901 agttttctaa gtcccaggta gaatggatga gtggtaaatt gatgagatgt tttgaagaca 961 aaagaaataa tccgtttcag tttcgccatc tctctttatg tcatggtctt tctgacttgg 1021 cccgtgtacc tagccctaaa gttgtacttg ccagccaacc tgacctggaa tgcggatttt 1081 caagggatct ctttattcag tggtgtcagg accctaaaaa ctcaatcatt ctaacctaca 1141 gaactactcc tgggacttta gcacgtttcc taattgataa tccttctgaa aaaattacag 1201 aaatagagtt gaggaaacgt gtgaagcttg aagggaaaga acttgaagaa tacttggaaa 1261 aagagaaact aaagaaagaa gctgccaaaa agcttgagca gtcaaaagag gcagatatag 1321 attccagtga tgagagtgat attgaggaag atattgacca gccatcagct cataagacga 1381 agcatgactt gatgatgaaa ggtgaaggca gtcgtaaagg aagttttttc aaacaggcaa 1441 aaaagtccta tcctatgttt cctgccccag aagaaagaat taaatgggat gaatatggag 1501 agattatcaa accagaggat ttcttagtgc cagagcttca agctactgaa gaagaaaaaa 1561 gcaaattaga atctggtttg acaaatggag atgaacctat ggatcaggat ttatctgatg 1621 ttcctactaa atgtatttct acaacagagt ctattgaaat aaaagcccgg gttacctaca 1681 tagactatga aggacgctct gatggggatt ccattaaaaa aatcattaat cagatgaaac 1741 cacgacagtt gatcatcgtc catggcccac cagaggccag tcaagatctg gcagagtgct 1801 gtcgcgcctt tggtgggaaa gatattaaag tgtacatgcc aaagctacat gaaacagttg 1861 atgccactag tgaaactcac atctaccagg tgaggttaaa agactcactt gtcagctctc 1921 ttcagttttg taaggcaaaa gatgctgaat tagcttggat agatggtgtc ttagatatga 1981 gagtttccaa agtggacaca ggggttattt tagaagaagg agaactaagg gatgatggag 2041 aagacTCAGA GATGCAAGTG GAAGCTCCCT CAGATTCTAG CGTTATAGCA CAACAAAAGG 2101 CCATGAAAAG TTTGTTCGGA GATGATGAAA AAGAAACAGG TGAAGAAAGT GAGATCATTC 2161 CTACTTTGGA ACCCTTGCCA CCTCATGAGG TTCCTGGACA TCAGTCAGTT TTTATGAATG 2221 AACCAAGGCT GTCAGACTTC AAGCAAGTTC TCTTACGGGA GGGAATTCAA GCTGAATTTG 2281 TAGGAGGTGT ACTTGTTTGC AACAATCAAG TAGCAGTCCG CAGAACGGAA ACTGGACGCA 2341 TTGGATTAGA AGGCTGCCTT TGTCAAGATT TTTATAGGAT AAGAGACCTT TTATATGAAC 2401 AATATGCCAT TGTATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2461 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2521 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CACAGGGTCA ACGAGCAAGT 2581 ATCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt