Construct: ORF TRCN0000476194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002393.1_s317c1
- Derived from:
- ccsbBroadEn_06964
- DNA Barcode:
- CCCAGCTCAACAACATTCATACTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SLC9A2 (6549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476194
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6549 | SLC9A2 | solute carrier family 9 mem... | NM_003048.6 | 99.9% | 69.5% | 1696G>T |
| 2 | mouse | 226999 | Slc9a2 | solute carrier family 9 (so... | NM_001033289.2 | 84.7% | 66.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1764
- ORF length:
- 1695
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaccactg ggcaactgga ggagcctgcg ggcgccactg cctccgatgc 121 tgttgctgct gctcctgcag gtggcggggc ccgtgggcgc cctggcggag accttgctga 181 acgcgccgag ggccatgggc accagttcca gcccgcctag ccctgcgagc gtggtggctc 241 ccggaacgac gctgttcgag gagagccggc tgcctgtgtt tacgctggat tacccccacg 301 tgcagatccc cttcgagatc accctttgga tcctgctggc ctccctggcc aagattggct 361 tccatctgta tcacaagttg cccacaatag tgcctgagag ctgccttctt ataatggttg 421 gacttctact aggtgggatt atttttggtg ttgatgagaa gtctccccct gcaatgaaga 481 ctgatgtatt tttcttgtac ctcctcccac ccatcgtgct ggatgccggc tatttcatgc 541 ccactcgccc attctttgag aacattggca cgattttctg gtatgctgtg gtagggacac 601 tttggaattc cattggcatt ggggtgtctt tgtttggtat ctgccagatc gaagcattcg 661 gcctcagcga catcactttg ctccagaacc tgctctttgg cagcttaatc tcagctgtcg 721 atcctgtggc tgtgcttgct gtctttgaga acattcacgt caatgagcag ctctacatcc 781 tggtctttgg agagtccctg ctgaatgatg cagtaacagt ggtcctgtac aacttgttca 841 agtcgttttg ccagatgaaa accattgaga ccattgatgt gtttgcagga atcgccaact 901 tctttgttgt gggaatcggt ggggtgctga ttggcatctt cttgggcttt atagcggcat 961 ttactactcg attcacccat aatatccgag tgatcgagcc actgtttgtt ttcctgtaca 1021 gttatttgtc ctacatcaca gctgaaatgt ttcacctctc aggcatcatg gcaatcactg 1081 cttgtgcaat gactatgaat aagtacgtag aagaaaatgt atctcagaaa tcctacacga 1141 ccatcaagta cttcatgaag atgctgagca gtgtcagcga aaccttgatc ttcatcttca 1201 tgggtgtgtc taccgtgggc aagaaccacg agtggaactg ggccttcgtc tgcttcaccc 1261 tggccttctg cctcatgtgg cgagccctgg gtgtttttgt cctgactcag gtcattaata 1321 ggttccggac cattcccctg acctttaagg accagttcat cattgcctat ggaggacttc 1381 gaggtgccat ctgttttgcg ttagtgtttc tccttcctgc tgctgtgttt cctcggaaaa 1441 aattgtttat tacggctgcc attgttgtca tattctttac tgtcttcatt ctgggaataa 1501 ctattcgacc actggtggag tttcttgatg tcaagaggtc caataagaaa caacaagctg 1561 tcagtgaaga aatctattgt cggttgtttg atcatgtgaa gactggaatt gaagatgttt 1621 gtggacattg gggtcacaac ttttggagag acaagtttaa gaagtttgat gataaatatc 1681 tgcggaagct tttgattcgg gaaaaccaac caaagtcaag tattgtatct ttatataaaa 1741 agcttgaaat aaaacatgcc atttagatgg cagagactgg gatgataagt actgtcccta 1801 catttgcatc tctaaatgat tgtcgtgaag aaaaaataag gaaggtcacg tccagtgaaa 1861 ctgatgaaat tcgagaactc ttatcaagaa atctctatca aatccgtcag cgaactttat 1921 cctacaacag acacagtctg acagccgaca caagtgagag acaagccaag gagattctga 1981 ttcgccggcg acacagtttg cgagaaagca ttaggaagga cagcagcttg aatcgagaac 2041 acagggcttc cacttcaacc tcccgatatt tatccttacc taaaaatacg aagcttccag 2101 aaaagctaca aaagaggagg actatttcta ttgcagatgg caatagcagc gactcagacg 2161 cagatgccgg gaccaccgtg ctcaatttgc agcccagagc caggcgcttc ttgccagaac 2221 agttctccaa gaaatccccc cagtcctata aaatggaatG GAAGAATGAG GTAGATGTTG 2281 ATTCTGGCCG AGATATGCCC AGCACCCCCC CAACACCCCA CAGCAGAGAA AAGGGCACCC 2341 AGACGTCAGG CTTACTACAG CAGCCCCTTC TCTCTAAAGA CCAGTCTGGC TCAGAGAGGG 2401 AAGACAGTTT GACTGAAGGC ATCCCGCCCA AGCCGCCACC ACGGCTGGTC TGGAGGGCAT 2461 CGGAACCTGG AAGCCGGAAA GCCCGATTTG GGAGTGAGAA GCCTTTGCCA ACTTTCTTGT 2521 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2581 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2641 GAAAGGACGA CCCAGCTCAA CAACATTCAT ACTTACGCGT TAAGTCgaca atcaacctct 2701 ggattacaaa atttgtgaaa gatt