Construct: ORF TRCN0000476231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003089.1_s317c1
- Derived from:
- ccsbBroadEn_11516
- DNA Barcode:
- TCCGTGCGGCCCGACTGCAAGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEBL (10529)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476231
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10529 | NEBL | nebulette | XM_005252344.5 | 79.3% | 77.3% | (many diffs) |
| 2 | human | 10529 | NEBL | nebulette | XM_005252343.5 | 74.8% | 72.9% | (many diffs) |
| 3 | human | 10529 | NEBL | nebulette | NM_006393.2 | 68.8% | 67.1% | (many diffs) |
| 4 | human | 10529 | NEBL | nebulette | XR_001746996.1 | 66.7% | (many diffs) | |
| 5 | human | 10529 | NEBL | nebulette | XM_011519291.2 | 66.6% | 64.3% | (many diffs) |
| 6 | human | 10529 | NEBL | nebulette | XM_017015468.1 | 66.6% | 64.3% | (many diffs) |
| 7 | human | 10529 | NEBL | nebulette | XM_005252342.5 | 65.6% | 63.9% | (many diffs) |
| 8 | human | 10529 | NEBL | nebulette | XR_001746995.2 | 50.7% | (many diffs) | |
| 9 | human | 10529 | NEBL | nebulette | XR_242691.5 | 19.2% | 1_1596del;2132G>T;3745_11135del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2217
- ORF length:
- 2148
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagggtccct gtatttgagg atataaaaga tgaaactgaa gaagaaaaga 121 taggggaaga agaaaatgaa gaagaccagg tcttctataa gcctgttatt gaagacttaa 181 gcatggaatt ggccagaaaa tgcacggaac tcattagcga tatccgttat aaagaagagt 241 ttaaaaagtc caaggataag tgtacatttg tgactgacag tcctatgcta aaccatgtaa 301 aaaatatcgg tgcttttatt tctgaggcaa aatacaaagg caccattaaa gctgaccttt 361 ctaattctct ttataagcgg atgccagcca caattgacag tgtttttgca ggagaagtta 421 cacagctcca gagtgaggtg gcctacaagc agaaacatga tgctgccaaa ggattctcag 481 attatgccca catgaaggag ccccctgagg ttaaacatgc catggaggtc aataaacacc 541 agagtaatat ttcttatagg aaagacgtgc aggacaccca cacgtacagt gcagaacttg 601 acctaccaga catcaagatg gcaacccaga tctctaagat cataagcaat gcagaataca 661 agaaaggaca aggaataatg aataaagagc ccgctgtaat tggaagacca gattttgaac 721 atgccgtgga agcttctaaa ctttctagtc aaattaaata caaagaaaaa tttgataatg 781 aaatgaagga taagaaacat cattacaatc ctcttgaaag tgcttctttt aggcagaatc 841 agcttgctgc tacactggcg agcaatgtga agtacaagaa agacattcaa aatatgcatg 901 atccagtttc agatctccca aatttgttgt ttttagacca tgttttgaaa gccagcaaaa 961 tgctcagcgg ccgagaatat aaaaagctct ttgaggaaaa caaaggaatg tatcattttg 1021 atgcagatgc tgtggaacat ctgcaccata aaggcaatgc cgtcctccaa agtcaggtga 1081 aatataaaga agaatatgag aaaaataagg gaaagccaat gcttgaattt gttgagacac 1141 catcatatca agcttcaaag gaggctcaaa agatgcaaag tgaaaaagtt tacaaagagg 1201 attttgagaa ggagattaaa ggaaggtcat cactggattt agacaagact ccagaatttt 1261 tacatgtaaa gtacatcacc aaccttctga gggagaaaga atataaaaaa gatttggaaa 1321 atgagataaa agggaaagga atggaactta attcagaagt tcttgatatc caaagagcaa 1381 agcgagcctc tgaaatggca agtgagaaag aatacaagaa agacctggag tcaataatta 1441 aagggaaagg aatgcaagct ggcactgaca cccttgaaat gcagcatgcc aagaaggctg 1501 cagagatagc gagtgagaaa gactataaaa gagatctgga gactgaaatt aaagggaaag 1561 ggatgcaggt gagcacagac actcttgatg tccagagagc taagaaagca tccgagatgg 1621 ccagccagaa acaatacaag aaggacttag aaaatgaaat taaagggaaa ggaatgcaag 1681 tgagcatgga tatcccagat atccttcgag ccaagaggac atctgaaatc tatagccaga 1741 gaaagtataa agatgaagca gagaagatgc tttctaacta ttctaccata gcagatactc 1801 cTGAAATTCA GAGAATTAAG ACAACTCAAC AAAACATTAG TGCGGTATTT TATAAGAAAG 1861 AAGTGGGAGC TGGCACTGCA GTGAAAGATA GCCCAGAGAT CGAACGAGTG AAGAAAAATC 1921 AGCAGAATAT TAGTTCAGTG AAATACAAAG AAGAGATTAA ACATGCAACA GCCATTTCTG 1981 ATCCTCCAGA ACTAAAGAGA GTTAAAGAAA ACCAGAAGAA CATCAGCAAT CTCCAGTATA 2041 AAGAGCAAAA CTACAAGGCC ACTCCGGTAA GCATGACCCC GGAGATAGAG AGAGTGAGGC 2101 GAAACCAGGA GCAGCTGAGT GCGGCAAGTC GTTTCTGTTT CCTTTTATAT TTTCTCAAAC 2161 ATAACATATT TTTATTTAAA AATATGCCCT TTGGAAGGCC ATTATCCTTA GCAAACTTGC 2221 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2281 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2341 ATATATCTTG TGGAAAGGAC GATCCGTGCG GCCCGACTGC AAGGGGACGC GTTAAGTCga 2401 caatcaacct ctggattaca aaatttgtga aagatt