Construct: ORF TRCN0000476266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002899.1_s317c1
- Derived from:
- ccsbBroadEn_13726
- DNA Barcode:
- ATCTAGTTGATTGCCCCTGCAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS18P9 (645958)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6222 | RPS18 | ribosomal protein S18 | NM_022551.3 | 46.4% | 45.3% | (many diffs) |
| 2 | human | 645958 | RPS18P9 | ribosomal protein S18 pseud... | NR_077247.1 | 22.2% | 1_703del;903T>C;926_993del | |
| 3 | mouse | 20084 | Rps18 | ribosomal protein S18 | NM_011296.2 | 41.4% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 315
- ORF length:
- 222
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGCCAGC CTGGTTCTTG AACAGACAGA 121 AGGATGTAAA GGATGGAAAA TATAGCCAGG TCCTAGCCAA TGGTCTGGAC AACAAGCTCT 181 GTGAAGACCT GGAGCGACTG AAAAAGATTC GGGCCCGTAG AGGGCTGCGT CACTTCTGGG 241 GCCTTCGTGT CCGAGGCCAG CACACCAAGA CCACAGGCTG CCGTGGCCGC ACCGTGGGTG 301 TGTCTAAGAA GAAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 361 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 421 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCTAGTTGA TTGCCCCTGC 481 AAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt