Construct: ORF TRCN0000476272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005382.1_s317c1
- Derived from:
- ccsbBroadEn_08470
- DNA Barcode:
- TTCTATGGGACTCGTTTACGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLFN12 (55106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476272
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55106 | SLFN12 | schlafen family member 12 | NM_001289009.2 | 99.8% | 99.6% | 129T>G;502T>C |
| 2 | human | 55106 | SLFN12 | schlafen family member 12 | NM_018042.5 | 99.8% | 99.6% | 129T>G;502T>C |
| 3 | human | 55106 | SLFN12 | schlafen family member 12 | XM_005257995.5 | 99.8% | 99.6% | 129T>G;502T>C |
| 4 | human | 55106 | SLFN12 | schlafen family member 12 | XM_011524967.3 | 99.8% | 99.6% | 129T>G;502T>C |
| 5 | human | 55106 | SLFN12 | schlafen family member 12 | XM_017024809.2 | 99.8% | 99.6% | 129T>G;502T>C |
| 6 | human | 55106 | SLFN12 | schlafen family member 12 | XM_017024810.2 | 99.8% | 99.6% | 129T>G;502T>C |
| 7 | human | 55106 | SLFN12 | schlafen family member 12 | XM_024450822.1 | 99.8% | 99.6% | 129T>G;502T>C |
| 8 | human | 100506736 | SLFN12L | schlafen family member 12 like | XM_024450523.1 | 82.6% | 73.2% | (many diffs) |
| 9 | human | 100506736 | SLFN12L | schlafen family member 12 like | NM_001195790.1 | 80.2% | 71% | (many diffs) |
| 10 | human | 100506736 | SLFN12L | schlafen family member 12 like | XM_017024006.1 | 80.2% | 71% | (many diffs) |
| 11 | human | 100506736 | SLFN12L | schlafen family member 12 like | XM_017024007.1 | 80.2% | 71% | (many diffs) |
| 12 | human | 100506736 | SLFN12L | schlafen family member 12 like | XM_024450520.1 | 80.2% | 71% | (many diffs) |
| 13 | human | 100506736 | SLFN12L | schlafen family member 12 like | XM_024450521.1 | 80.2% | 71% | (many diffs) |
| 14 | human | 100506736 | SLFN12L | schlafen family member 12 like | XM_024450522.1 | 80.2% | 71% | (many diffs) |
| 15 | human | 100506736 | SLFN12L | schlafen family member 12 like | NM_001363830.1 | 76.5% | 67.8% | (many diffs) |
| 16 | human | 55106 | SLFN12 | schlafen family member 12 | XM_017024811.2 | 66.6% | 65.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1800
- ORF length:
- 1734
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaataag 61 ttggcatgaa catcagtgtt gatttggaaa cgaattatgc cgagttggtt ctagatgtgg 121 gaagagtcac tcttggagag aacagtagga aaaaaatgaa ggattgtaaa ctgagaaaaa 181 agcagaatga aagggtctca cgagctatgt gtgctctgct caattctgga gggggagtga 241 tcaaggctga aattgagaat gaagactata gttatacaaa agatggaata ggactagatt 301 tggaaaattc ttttagtaac attctgttat ttgttcctga gtacttagac ttcatgcaga 361 atggtaacta ctttctgatt tttgtgaagt catggagctt gaacacctct ggtctgcgga 421 ttaccacctt gagctccaat ttgtacaaaa gagatataac atctgcaaaa gtcatgaatg 481 ccactgctgc actggagttc ctcaaagaca tgaaaaagac tagagggaga ttgtatttaa 541 gaccagaatt gctggcaaag aggccccgtg ttgatataca agaagaaaat aacatgaagg 601 ccttggccgg ggtttttttt gatagaacag aacttgatcg gaaagaaaaa ttgaccttta 661 ctgaatccac acatgttgaa attaaaaact tctcgacaga aaagttgtta caacgaatta 721 aagagattct ccctcaatat gtttctgcat ttgcaaatac tgatggagga tatttgttca 781 ttggtttaaa tgaagataaa gaaataattg gctttaaagc agagatgagt gacctcgatg 841 acttagaaag agaaatcgaa aagtccatta ggaagatgcc tgtgcatcac ttctgtatgg 901 agaagaagaa gataaattat tcatgcaaat tccttggagt atatgataaa ggaagtcttt 961 gtggatatgt ctgtgcactc agagtggagc gcttctgctg tgcagtgttt gctaaagagc 1021 ctgattcctg gcatgtgaaa gataaccgtg tgatgcagtt gaccaggaag gaatggatcc 1081 agttcatggt ggaggctgaa ccaaaatttt ccagttcata tgaagaggtg atctctcaaa 1141 taaatacgtc attacctgct ccccacagtt ggcctctttt ggaatggcaa cggcagagac 1201 atcactgtcc agggctatca ggaaggataa cgtatactcc agaaaacctt tgcagaaaac 1261 tgttcttaca acatgaagga cttaagcaat taatatgtga agaaatggac tctgtcagaa 1321 agggctcact gatcttctct aggagctggt ctgtggatct gggcttgcaa gagaaccaca 1381 aagtcctctg tgatgctctt ctgatttccc aggacagtcc tccagtccta tacaccttcc 1441 acatggtaca ggatgaggag tttaaaggct attctacaca aactgcccta aCCTTAAAGC 1501 AGAAGCTGGC AAAAATTGGT GGTTACACTA AAAAAGTGTG TGTCATGACA AAGATCTTCT 1561 ACTTGAGCCC TGAAGGCATG ACAAGCTGCC AGTATGATTT AAGGTCGCAA GTAATTTACC 1621 CTGAATCCTA CTATTTTACA AGAAGGAAAT ACTTGCTGAA AGCCCTTTTT AAAGCCTTAA 1681 AGAGACTCAA GTCTCTGAGA GACCAGTTTT CCTTTGCAGA AAATCTATAC CAGATAATCG 1741 GTATAGATTG CTTTCAGAAG AATGATAAAA AGATGTTTAA ATCTTGTCGA AGGCTCACCT 1801 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1861 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1921 TTTATATATC TTGTGGAAAG GACGATTCTA TGGGACTCGT TTACGGAACA CGCGTTAAGT 1981 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt