Construct: ORF TRCN0000476275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010519.1_s317c1
- Derived from:
- ccsbBroadEn_08834
- DNA Barcode:
- TTGTCACTTTTATACGGGTAGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPFFR1 (64106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64106 | NPFFR1 | neuropeptide FF receptor 1 | NM_022146.5 | 99.9% | 100% | 597G>T |
| 2 | mouse | 237362 | Npffr1 | neuropeptide FF receptor 1 | NM_001177511.1 | 86% | 86.5% | (many diffs) |
| 3 | mouse | 237362 | Npffr1 | neuropeptide FF receptor 1 | XM_011243444.2 | 62.5% | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1359
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagggggag ccctcccagc ctcccaacag cagttggccc ctaagtcaga 121 atgggactaa cactgaggcc accccggcta caaacctcac cttctcctcc tactatcagc 181 acacctcccc tgtggcggcc atgttcattg tggcctatgc gctcatcttc ctgctctgca 241 tggtgggcaa caccctggtc tgtttcatcg tgctcaagaa ccggcacatg catactgtca 301 ccaacatgtt catcctcaac ctggctgtca gtgacctgct ggtgggcatc ttctgcatgc 361 ccaccaccct tgtggacaac ctcatcactg ggtggccctt cgacaatgcc acatgcaaga 421 tgagcggctt ggtgcagggc atgtctgtgt cggcttccgt tttcacactg gtggccattg 481 ctgtggaaag gttccgctgc atcgtgcacc ctttccgcga gaagctgacc ctgcggaagg 541 cgctcgtcac catcgccgtc atctgggccc tggcgctgct catcatgtgt ccctcggccg 601 tcacgctgac cgtcacccgt gaggagcacc acttcatggt ggacgcccgc aaccgctcct 661 accctctcta ctcctgctgg gaggcctggc ccgagaaggg catgcgcagg gtctacacca 721 ctgtgctctt ctcgcacatc tacctggcgc cgctggcgct catcgtggtc atgtacgccc 781 gcatcgcgcg caagctctgc caggccccgg gcccggcccc cgggggcgag gaggctgcgg 841 acccgcgagc atcgcggcgc agagcgcgcg tggtgcacat gctggtcatg gtggcgctgt 901 tcttcacgct gtcctggctg ccgctctggg cgctgctgct gctcatcgac tacgggcagc 961 tcagcgcgcc gcagctgcac ctggtcaccg tctacgcctt ccccttcgcg cactggctgg 1021 CCTTCTTCAA CAGCAGCGCC AACCCCATCA TCTACGGCTA CTTCAACGAG AACTTCCGCC 1081 GCGGCTTCCA GGCCGCCTTC CGCGCCCGCC TCTGCCCGCG CCCGTCGGGG AGCCACAAGG 1141 AGGCCTACTC CGAGCGGCCC GGCGGGCTTC TGCACAGGCG GGTCTTCGTG GTGGTGCGGC 1201 CCAGCGACTC CGGGCTGCCC TCTGAGTCGG GCCCTAGCAG TGGGGCCCCC AGGCCCGGCC 1261 GCCTCCCGCT GCGGAATGGG CGGGTGGCTC ACCACGGCTT GCCCAGGGAA GGGCCTGGCT 1321 GCTCCCACCT GCCCCTCACC ATTCCAGCCT GGGATATCTT GCCAACTTCC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGATTGTCA CTTTTATACG GGTAGATGAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt