Construct: ORF TRCN0000476276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002843.1_s317c1
- Derived from:
- ccsbBroadEn_08398
- DNA Barcode:
- CCCCGTCAAATAACTTGGATCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT1A4 (54657)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54657 | UGT1A4 | UDP glucuronosyltransferase... | NM_007120.3 | 99.9% | 100% | 471T>C |
| 2 | human | 54579 | UGT1A5 | UDP glucuronosyltransferase... | NM_019078.1 | 96.3% | 93.4% | (many diffs) |
| 3 | human | 54659 | UGT1A3 | UDP glucuronosyltransferase... | NM_019093.2 | 96.3% | 93.2% | (many diffs) |
| 4 | human | 54578 | UGT1A6 | UDP glucuronosyltransferase... | NM_205862.2 | 48.9% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1671
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagagga ctccaggttc ccctgccgcg gctggccaca ggactgctgc 121 tcctcctcag tgtccagccc tgggctgaga gtggaaaggt gttggtggtg cccactgatg 181 gcagcccctg gctcagcatg cgggaggcct tgcgggagct ccatgccaga ggccaccagg 241 cggtggtcct caccccagag gtgaatatgc acatcaaaga agagaaattt ttcaccctga 301 cagcctatgc tgttccatgg acccagaagg aatttgatcg cgttacgctg ggctacactc 361 aagggttctt tgaaacagaa catcttctga agagatattc tagaagtatg gcaattatga 421 acaatgtatc tttggccctt cataggtgtt gtgtggagct actgcataat gaggccctga 481 tcaggcacct gaatgctact tcctttgatg tggttttaac agaccccgtt aacctctgcg 541 gggcggtgct ggctaagtac ctgtcgattc ctgctgtgtt tttttggagg tacattccat 601 gtgacttaga ctttaagggc acacagtgtc caaatccttc ctcctatatt cctaagttac 661 taacgaccaa ttcagaccac atgacattcc tgcaaagggt caagaacatg ctctaccctc 721 tggccctgtc ctacatttgc catacttttt ctgcccctta tgcaagtctt gcctctgagc 781 tttttcagag agaggtgtca gtggtggatc ttgtcagcta tgcatccgtg tggctgttcc 841 gaggggactt tgtgatggac taccccaggc cgatcatgcc caacatggtc ttcattgggg 901 gcatcaactg tgccaacggg aagccactat ctcaggaatt tgaagcctac attaatgctt 961 ctggagaaca tggaattgtg gttttctctt tgggatcaat ggtctcagaa attccagaga 1021 agaaagctat ggcaattgct gatgctttgg gcaaaatccc tcagacagtc ctgtggcggt 1081 acactggaac ccgaccatcg aatcttgcga acaacacgat acttgttaag tggctacccc 1141 aaaacgatct gcttggtcac ccgatgaccc gtgcctttat cacccatgct ggttcccatg 1201 gtgtttatga aagcatatgc aatggcgttc ccatggtgat gatgcccttg tttggtgatc 1261 agatggacaa tgcaaagcgc atggagacta agggagctgg agtgacccTG AATGTTCTGG 1321 AAATGACTTC TGAAGATTTA GAAAATGCTC TAAAAGCAGT CATCAATGAC AAAAGTTACA 1381 AGGAGAACAT CATGCGCCTC TCCAGCCTTC ACAAGGACCG CCCGGTGGAG CCGCTGGACC 1441 TGGCCGTGTT CTGGGTGGAG TTTGTGATGA GGCACAAGGG CGCGCCACAC CTGCGCCCCG 1501 CAGCCCACGA CCTCACCTGG TACCAGTACC ATTCCTTGGA CGTGATTGGT TTCCTCTTGG 1561 CCGTCGTGCT GACAGTGGCC TTCATCACCT TTAAATGTTG TGCTTATGGC TACCGGAAAT 1621 GCTTGGGGAA AAAAGGGCGA GTTAAGAAAG CCCACAAATC CAAGACCCAT TTGCCAACTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGACCCC GTCAAATAAC TTGGATCGCT ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt