Construct: ORF TRCN0000476296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003755.1_s317c1
- Derived from:
- ccsbBroadEn_11627
- DNA Barcode:
- GATGGCCTCCGTGTAGCACCTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XRN2 (22803)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | XM_017027723.2 | 93.9% | 93.9% | 0_1ins102 |
| 2 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | NM_012255.5 | 58.9% | 58.9% | 1_1170del |
| 3 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | NM_001317960.1 | 54.4% | 54.4% | 1_1404del |
| 4 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | XM_006499451.2 | 84.4% | 87.8% | (many diffs) |
| 5 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | XM_006499450.3 | 64.5% | 67.1% | (many diffs) |
| 6 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | NM_011917.2 | 53.3% | 55.4% | (many diffs) |
| 7 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | XM_011239519.2 | 37.1% | 37.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1746
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat catgttagca gttggtgaag ttgaggatag catttttaaa aagagaaagg 121 atgatgagga cagttttaga agacgacaga aagaaaaaag aaagagaatg aagagagatc 181 aaccagcttt cactcctagt ggaatattaa ctcctcatgc cttgggttca agaaattcac 241 caggttctca agtagccagt aatccgagac aagcagccta tgaaatgagg atgcagaata 301 actctagtcc ttcgatatct cctaatacga gtttcacatc tgatggctcc ccgtctccat 361 taggaggaat taagcgaaaa gcagaagaca gtgacagtga acctgagcca gaggataatg 421 tcaggttatg ggaagctggc tggaagcagc ggtactacaa gaacaaattt gatgtggatg 481 cagctgatga gaaattccgt cggaaagttg tgcagtcgta cgttgaagga ctttgctggg 541 ttcttagata ttattaccag ggctgtgctt cctggaagtg gtattatcca tttcattatg 601 caccatttgc ttcagacttt gaaggcattg cagacatgcc atctgatttt gagaagggta 661 cgaaaccgtt taaaccacta gaacaactta tgggggtatt tccagctgca agtggtaatt 721 ttctacctcc atcatggcgg aagctcatga gtgatcctga ttctagtata attgacttct 781 atcctgaaga ttttgctatt gatttgaatg ggaagaaata tgcatggcaa ggtgttgctc 841 tcttgccatt cgtggatgag cgaaggctac gagctgccct agaagaggta tacccagacc 901 tcactccaga agagaccaga agaaacagcc ttggaggtga tgtcttattt gtggggaaac 961 atcacccact ccatgacttc attttagagc tgtaccagac aggttccaca gagccagtgg 1021 aggtaccccc tgaactatgt catgggattc aaggaaagtt ttctttggat gaagaagcca 1081 ttcttccaga tcaaatagta tgttctcctg ttcctatgtt aagggatctg acacagaaca 1141 ctgtagtcag tattaatttt aaagacccac agtttgctga agattacatt tttaaagctg 1201 taatgcttcc aggagcaaga aagccagcag cagtactgaa acctagtgac tgggaaaaat 1261 ccagcaatgg acggcagtgg aagcctcagc ttggctttaa ccgtgaccgg aggcctgtgc 1321 acctggatca ggcagccttc aggactttgg gccatgtgat gccaagaggc TCAGGAACTG 1381 GCATTTACAG CAATGCTGCA CCACCACCTG TGACTTACCA GGGAAACTTA TACAGGCCGC 1441 TTTTGAGAGG ACAAGCCCAG ATTCCAAAAC TTATGTCAAA TATGAGGCCC CAGGATTCCT 1501 GGCGAGGTCC TCCTCCCCTT TTCCAGCAGC AAAGGTTTGA CAGAGGCGTT GGGGCTGAAC 1561 CTCTGCTCCC ATGGAACCGG ATGCTGCAAA CCCAGAATGC AGCCTTCCAG CCAAACCAGT 1621 ACCAGATGCT AGCTGGGCCT GGTGGGTATC CACCCAGACG AGATGATCGT GGAGGGAGAC 1681 AGGGATATCC CAGAGAAGGA AGGAAATACC CTTTGCCACC ACCCTCAGGA AGATACAATT 1741 GGAATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1801 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1861 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGATGGCCTC CGTGTAGCAC CTGAAACGCG 1921 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt