Transcript: Human XM_017027723.2

PREDICTED: Homo sapiens 5'-3' exoribonuclease 2 (XRN2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
XRN2 (22803)
Length:
3450
CDS:
1383..2963

Additional Resources:

NCBI RefSeq record:
XM_017027723.2
NBCI Gene record:
XRN2 (22803)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027723.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000119961 GCTATTACATAGCTGATCGTT pLKO.1 610 5UTR 100% 3.000 4.200 N Xrn2 n/a
2 TRCN0000049902 CCGTCTCCATTAGGAGGAATT pLKO.1 1566 CDS 100% 0.000 0.000 N XRN2 n/a
3 TRCN0000293639 TACATAGCTGATCGTTTAAAT pLKO_005 615 5UTR 100% 15.000 10.500 N XRN2 n/a
4 TRCN0000339955 TACATAGCTGATCGTTTAAAT pLKO_005 615 5UTR 100% 15.000 10.500 N Xrn2 n/a
5 TRCN0000293611 TGATCCTGATTCTAGTATAAT pLKO_005 1967 CDS 100% 15.000 10.500 N XRN2 n/a
6 TRCN0000049898 CCACACATGAACCGAACTTTA pLKO.1 811 5UTR 100% 13.200 9.240 N XRN2 n/a
7 TRCN0000349677 CCACACATGAACCGAACTTTA pLKO_005 811 5UTR 100% 13.200 9.240 N XRN2 n/a
8 TRCN0000293640 GTGTATTCTAGATCATCTAAG pLKO_005 3259 3UTR 100% 10.800 7.560 N XRN2 n/a
9 TRCN0000119959 CCAAATGATGTGGAGTTTGAT pLKO.1 213 5UTR 100% 5.625 3.938 N Xrn2 n/a
10 TRCN0000339918 CCAAATGATGTGGAGTTTGAT pLKO_005 213 5UTR 100% 5.625 3.938 N Xrn2 n/a
11 TRCN0000049901 CCAGAGGATAATGTCAGGTTA pLKO.1 1623 CDS 100% 4.950 3.465 N XRN2 n/a
12 TRCN0000049900 CGTGAGTATTTGGAAAGAGAA pLKO.1 1014 5UTR 100% 4.950 3.465 N XRN2 n/a
13 TRCN0000319294 CGTGAGTATTTGGAAAGAGAA pLKO_005 1014 5UTR 100% 4.950 3.465 N XRN2 n/a
14 TRCN0000049899 CCCTGAACTATGTCATGGGAT pLKO.1 2243 CDS 100% 2.640 1.848 N XRN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027723.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11627 pDONR223 100% 93.9% 93.9% None 0_1ins102 n/a
2 ccsbBroad304_11627 pLX_304 0% 93.9% 93.9% V5 0_1ins102 n/a
3 TRCN0000476296 GATGGCCTCCGTGTAGCACCTGAA pLX_317 22.7% 93.9% 93.9% V5 0_1ins102 n/a
4 ccsbBroadEn_14067 pDONR223 100% 55.3% 32.8% None 0_1ins1272;925_926insA n/a
5 ccsbBroad304_14067 pLX_304 0% 55.3% 32.8% V5 (not translated due to prior stop codon) 0_1ins1272;925_926insA n/a
6 TRCN0000474266 GGCGTTAGAGCCTGAGAGGTGTGG pLX_317 13.4% 55.3% 32.8% V5 (not translated due to prior stop codon) 0_1ins1272;925_926insA n/a
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