Construct: ORF TRCN0000476301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017693.1_s317c1
- Derived from:
- ccsbBroadEn_10161
- DNA Barcode:
- TTGAAACACCAATGATCCTCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG11 (440138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440138 | ALG11 | ALG11 alpha-1,2-mannosyltra... | NM_001004127.3 | 99.9% | 99.7% | 287C>T |
| 2 | mouse | 207958 | Alg11 | asparagine-linked glycosyla... | NM_001243161.1 | 85.7% | 85.9% | (many diffs) |
| 3 | mouse | 207958 | Alg11 | asparagine-linked glycosyla... | XM_006509045.2 | 82% | 82.3% | (many diffs) |
| 4 | mouse | 207958 | Alg11 | asparagine-linked glycosyla... | NM_183142.4 | 78.3% | 78.6% | (many diffs) |
| 5 | mouse | 207958 | Alg11 | asparagine-linked glycosyla... | NR_040650.1 | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1545
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcactat ggcggccggc gaaaggagct ggtgcctgtg caagttgttg aggttttttt 121 attcattatt cttccctggg ctcattgtat gtggaacttt atgtgtgtgt ttggtcattg 181 tcctttgggg aatcagactg ctgctacaga gaaagaaaaa attagtgtca actagcaaaa 241 atgggaaaaa tcaaatggtg attgcatttt ttcatccata ctgcaatgct ggtggaggag 301 gagaaagagt tttatggtgt gctttaagag ccctgcagaa aaagtatcct gaagtagttt 361 atgttgttta taccggcgat gttaatgtca acggtcaaca gatactagaa ggtgctttca 421 gaagatttaa catcagatta attcacccag tgcagtttgt ttttttaagg aaacgctatc 481 ttgtggaaga ttcactgtat cctcacttca cactgctggg ccaaagtcta ggatccattt 541 ttcttggctg ggaagctcta atgcagtgtg ttcctgatgt ttacattgat tcaatgggat 601 acgcttttac gcttcctctg tttaagtata tagggggttg ccaagttgga agctatgttc 661 attatcctac tatcagcacc gacatgctct ctgtagtgaa gaatcaaaat attggattta 721 ataatgcagc cttcattacc aggaatcctt ttctcagcaa agtaaagctc atctactact 781 atttatttgc ttttatttat ggacttgttg gttcttgcag tgatgtagtc atggtcaatt 841 cttcttggac actaaaccat attctctcac tatggaaagt tgggaattgc actaacattg 901 tttatccacc ttgtgatgtg cagacatttc tggacattcc cttacatgag aaaaagatga 961 ccccaggaca tttgctggtt tctgttggcc agtttaggcc ggaaaagaat catccattgc 1021 agatcagagc ctttgctaaa ttgctgaata agaagatggt tgagtcacct ccttcgctta 1081 aacttgtcct cattggaggt tgtcgtaaca aagatgatga acttagggta aaccaactga 1141 gaaggctgtc tgaggattta ggagttcaag aatatgtgga atttaaaata aaCATTCCAT 1201 TTGATGAATT AAAGAATTAT TTGTCTGAAG CAACAATTGG TCTGCATACC ATGTGGAACG 1261 AGCATTTTGG GATTGGAGTT GTGGAGTGTA TGGCAGCTGG CACAATTATC CTTGCACACA 1321 ATTCGGGGGG CCCAAAGCTT GACATTGTGG TTCCTCACGA AGGAGATATA ACTGGCTTTC 1381 TGGCTGAGAG TGAAGAAGAC TATGCTGAAA CTATCGCTCA CATTCTTTCC ATGTCTGCAG 1441 AAAAGAGACT CCAAATCAGA AAAAGTGCTC GTGCATCTGT AAGCAGATTC TCTGATCAGG 1501 AATTTGAAGT GACATTCCTA TCATCTGTGG AAAAGTTATT TAAGTTGCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TTGAAACACC AATGATCCTC ACACACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt