Construct: ORF TRCN0000476301

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017693.1_s317c1
Derived from:
ccsbBroadEn_10161
DNA Barcode:
TTGAAACACCAATGATCCTCACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG11 (440138)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476301
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440138 ALG11 ALG11 alpha-1,2-mannosyltra... NM_001004127.3 99.9% 99.7% 287C>T
2 mouse 207958 Alg11 asparagine-linked glycosyla... NM_001243161.1 85.7% 85.9% (many diffs)
3 mouse 207958 Alg11 asparagine-linked glycosyla... XM_006509045.2 82% 82.3% (many diffs)
4 mouse 207958 Alg11 asparagine-linked glycosyla... NM_183142.4 78.3% 78.6% (many diffs)
5 mouse 207958 Alg11 asparagine-linked glycosyla... NR_040650.1 25.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1545
ORF length:
1476
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcactat ggcggccggc gaaaggagct ggtgcctgtg caagttgttg aggttttttt
121attcattatt cttccctggg ctcattgtat gtggaacttt atgtgtgtgt ttggtcattg
181tcctttgggg aatcagactg ctgctacaga gaaagaaaaa attagtgtca actagcaaaa
241atgggaaaaa tcaaatggtg attgcatttt ttcatccata ctgcaatgct ggtggaggag
301gagaaagagt tttatggtgt gctttaagag ccctgcagaa aaagtatcct gaagtagttt
361atgttgttta taccggcgat gttaatgtca acggtcaaca gatactagaa ggtgctttca
421gaagatttaa catcagatta attcacccag tgcagtttgt ttttttaagg aaacgctatc
481ttgtggaaga ttcactgtat cctcacttca cactgctggg ccaaagtcta ggatccattt
541ttcttggctg ggaagctcta atgcagtgtg ttcctgatgt ttacattgat tcaatgggat
601acgcttttac gcttcctctg tttaagtata tagggggttg ccaagttgga agctatgttc
661attatcctac tatcagcacc gacatgctct ctgtagtgaa gaatcaaaat attggattta
721ataatgcagc cttcattacc aggaatcctt ttctcagcaa agtaaagctc atctactact
781atttatttgc ttttatttat ggacttgttg gttcttgcag tgatgtagtc atggtcaatt
841cttcttggac actaaaccat attctctcac tatggaaagt tgggaattgc actaacattg
901tttatccacc ttgtgatgtg cagacatttc tggacattcc cttacatgag aaaaagatga
961ccccaggaca tttgctggtt tctgttggcc agtttaggcc ggaaaagaat catccattgc
1021agatcagagc ctttgctaaa ttgctgaata agaagatggt tgagtcacct ccttcgctta
1081aacttgtcct cattggaggt tgtcgtaaca aagatgatga acttagggta aaccaactga
1141gaaggctgtc tgaggattta ggagttcaag aatatgtgga atttaaaata aaCATTCCAT
1201TTGATGAATT AAAGAATTAT TTGTCTGAAG CAACAATTGG TCTGCATACC ATGTGGAACG
1261AGCATTTTGG GATTGGAGTT GTGGAGTGTA TGGCAGCTGG CACAATTATC CTTGCACACA
1321ATTCGGGGGG CCCAAAGCTT GACATTGTGG TTCCTCACGA AGGAGATATA ACTGGCTTTC
1381TGGCTGAGAG TGAAGAAGAC TATGCTGAAA CTATCGCTCA CATTCTTTCC ATGTCTGCAG
1441AAAAGAGACT CCAAATCAGA AAAAGTGCTC GTGCATCTGT AAGCAGATTC TCTGATCAGG
1501AATTTGAAGT GACATTCCTA TCATCTGTGG AAAAGTTATT TAAGTTGCCA ACTTTCTTGT
1561ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1621AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1681GAAAGGACGA TTGAAACACC AATGATCCTC ACACACGCGT TAAGTCgaca atcaacctct
1741ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)