Construct: ORF TRCN0000476305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014807.1_s317c1
- Derived from:
- ccsbBroadEn_05415
- DNA Barcode:
- AGCAGAGGGTAGTGAACCTATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP47 (286464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286464 | CFAP47 | cilia and flagella associat... | XM_017029453.1 | 26% | 26% | 1_4275del |
| 2 | human | 286464 | CFAP47 | cilia and flagella associat... | XM_017029452.1 | 17.7% | 16% | (many diffs) |
| 3 | human | 286464 | CFAP47 | cilia and flagella associat... | NM_001304548.2 | 14.7% | 13.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1575
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaattcat ctggataagc aaaacattat tttaaagaat gataaggatg 121 aatatcttaa gaagactaga gatggtgttt tgcctcccta ccaggatgct aaaccaccct 181 ctcctgcttc aattaaaaag acatacacca ctagcaaatt caatgatgct gaacctgcaa 241 agggaaactt atttattgga gtggaagtac tgcctgaaaa tttgcacttg gatgaaagtg 301 aaacatcaga ggaagatcat gggtctctgg aaaaggaaaa atatgaacaa ttcctttctc 361 ttgaggaagg aacaaaggca cactactttt ttgagaaggt tgtaaatgca gcacagacct 421 ggttcagtct ctttggctgg cctgaaggac cccattcttt ttctattcca gagactataa 481 gaagggatgt atataaaatg caattctact catcaacctc gccaccccaa aagttttcca 541 gacagaatga cttttccaaa tataataaga ccatttatga tgtgctgctc catttgagtg 601 gaaaaatgcc acctggaatt aattcaagtc aatctttacc tgtagataac catgaaaaaa 661 gggtaattca actccatttg caacattcct cacttctgga ctttctcaat gctcaaggag 721 gatgtatttc acatgtcctg ccagaatttt tgcttgaacc agaagattat aagaggtgga 781 ttgaaattat gtcttccact aatacgatgc ctgtgagttc ctgtacacct aagaaaaaat 841 gttctattgt tatagaaatg tctaaatttg aagcctggag taaacgggca tggacagatg 901 tatttctaca gatatacaag gttttggttc tatcccgtgt agtgccatac tgcagcaata 961 atatgccccc catatgtgtg caaaatacac caaaagtcaa tccttgtttt gcatccagca 1021 acatatattc tgattctgaa agaattttgc ttagttggat gaacataaat tatgagaata 1081 ctcgacatgt gatatggaaa aactgtcaca aagatgttat cccttctgag agatggatag 1141 taaattttga caaagacctt tcagatggtc ttgtttttgc aacacagttg ggagcctatt 1201 gcccattctt gattgagtct cattttataa atatgtacac acgaccaaaa agtcctgaag 1261 agtatctgca caattgcctg attattgtaa atactcttta tgaaattgac tttgacgtgg 1321 aaatacaggt gtgtctgagg gtgtttccaa aggagatgga tgcaggagtC AGTGGCCTGA 1381 GAAAGGAAGA TATGCCCTCA GTGTGGGTTG TTACCATCCG ATTGGCTGTG GCAGTGGCCA 1441 GAACAAAGAG ACAGAAGAAG GGGGATATTC AGTTTGCTTG TTTTTTTGTT TGTTTGTTCT 1501 TTTTTATTTT TGTCGTGATC TCTTTCTCCC TTTGGAGCAG GATGCATTTT TTTCTCCTTC 1561 TGCCCTTGGA CATCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA TTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGCAGAGGGT AGTGAACCTA 1741 TGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt