Construct: ORF TRCN0000476333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007661.1_s317c1
- Derived from:
- ccsbBroadEn_00519
- DNA Barcode:
- AATCATCTTCAATAAGTCTGTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETFB (2109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2109 | ETFB | electron transfer flavoprot... | NM_001985.3 | 100% | 100% | |
| 2 | human | 2109 | ETFB | electron transfer flavoprot... | XM_024451418.1 | 85.4% | 85.4% | 0_1ins111 |
| 3 | human | 2109 | ETFB | electron transfer flavoprot... | NM_001014763.1 | 72.1% | 69.3% | (many diffs) |
| 4 | mouse | 110826 | Etfb | electron transferring flavo... | NM_026695.3 | 87.7% | 92.1% | (many diffs) |
| 5 | mouse | 110826 | Etfb | electron transferring flavo... | NR_075104.1 | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 834
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggagctg cgcgtgctcg tagctgtcaa gagggtcatc gactacgccg 121 tgaagatccg agtgaagcct gacaggaccg gtgtggtcac ggatggtgtg aagcactcca 181 tgaacccctt ctgtgagatc gcggtggagg aggctgtgcg gctcaaggag aagaagctgg 241 tgaaggaggt catcgccgtc agctgtgggc ctgcacagtg ccaggagacg attcgtaccg 301 ccctggccat gggtgcagac cgaggtatcc acgtggaggt gcccccagca gaagcagaac 361 gcttgggtcc cctgcaggtg gctcgggtcc tggccaagct ggcagagaag gagaaggtgg 421 acctggtgct gctgggcaaa caggccatcg atgatgactg taaccagaca gggcagatga 481 cagctggatt tcttgactgg ccacagggca cattcgccTC CCAGGTGACG CTGGAGGGGG 541 ACAAGTTGAA AGTGGAGCGG GAGATCGATG GGGGCCTGGA GACCCTGCGC CTGAAGCTGC 601 CAGCTGTGGT GACAGCTGAC CTGAGGCTCA ACGAGCCCCG CTACGCCACG CTGCCCAACA 661 TCATGAAAGC CAAGAAGAAG AAGATCGAGG TGATCAAGCC TGGGGACCTG GGTGTGGACC 721 TGACCTCCAA GCTCTCTGTG ATCAGTGTGG AGGACCCGCC CCAGCGCACG GCCGGCGTCA 781 AGGTGGAGAC CACTGAGGAC CTGGTGGCCA AGCTGAAGGA GATTGGGCGG ATTTTGCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAA ATCATCTTCA ATAAGTCTGT ACCACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att