Construct: ORF TRCN0000476357
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009348.1_s317c1
- Derived from:
- ccsbBroadEn_14112
- DNA Barcode:
- CGGACGGCTCCCGCTGATGTTAGA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF285 (26974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476357
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291490.2 | 99.9% | 99.3% | 1297delG |
| 2 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291491.2 | 99.9% | 99.3% | 1297delG |
| 3 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291489.2 | 73.6% | 73.2% | 1_465del;1762delG |
| 4 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_152354.6 | 73.6% | 73.2% | 1_465del;1762delG |
| 5 | human | 26974 | ZNF285 | zinc finger protein 285 | NM_001291488.2 | 72.8% | 72.3% | 1_486del;1783delG |
| 6 | human | 646915 | ZNF806 | zinc finger protein 806 | NM_001304449.1 | 69.6% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1374
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgagccc cagaactctc agggaagata taagggaatt tacatggaag 121 agaaattgta cagacgtgct cagcatgatg acagcctcag ttggacctca tgtgatcatc 181 atgagtccca agaatgtaaa ggagaggacc ctggtagaca tcccagctgt gggaaaaact 241 tgggtatgaa atcaacggtt gaaaaacgta atgcggccca tgtattacca cagcctttcc 301 catgtaataa ctgtggggtg gcctttgcag atgatacaga tcctcatgtc catcacagca 361 ctcacctagg agaaaaatct tataaatgtg accagtatgg aaagaacttt agtcagagcc 421 aagatcttat cgttcattgt aaaactcact ctggcaagac tccctatgaa ttccacgaat 481 ggcctatggg ctgcaaacag agctcagacc ttcccagata tcagaaagtc tcctcaggag 541 acaaacccta caaatgtaaa gaatgtggca agggcttcag gcgcagctct tcccttcaca 601 accatcatcg agtccacaca ggggagatgc cctacaaatg cgatgaatgt gggaaagggt 661 ttggatttag gtcacttctt tgtattcatc agggagtaca cacagggaaa aagccctata 721 aatgtgaaga gtgtgggaag ggctttgatc agagctccaa ccttcttgtc catcagagag 781 tccacactgg agagaagccc tacaaatgca gtgagtgtgg caagtgcttt agttcaagct 841 ccgttcttca agtccactgg aggtttcaca caggggagaa accatatagg tgtggtgagt 901 gtggaaaggg cttcagccaa tgtacacacc ttcacattca ccagagagtc cacacagggg 961 agaaaccata caaatgcaat gtgtgtggaa aggattttgc gtatagctct gttcttcaca 1021 ctcaTCAGAG AGTTCACACT GGAGAAAAAC CATATAAATG TGAAGTGTGT GGAAAGTGCT 1081 TCAGTTACAG TTCATATTTT CACTTACATC AAAGAGATCA CATCAGAGAG AAACCATATA 1141 AATGTGATGA GTGTGGTAAA GGCTTCAGCC GGAATTCAGA TCTTAATGTT CACCTCAGAG 1201 TCCACACAGG AGAGAGGCCC TATAAGTGTA AGGCATGTGG TAAGGGCTTC AGTCGTAATT 1261 CATACCTCCT TGCCCATCAG AGAGTGCATA TAGATGAGAC ACAGTACACA CACTGTGAGC 1321 GTGGAAAGGA CCTTCTGACT CATCAAAGAC TACATGAGCA GAGAAAACAT TATTGCCAAC 1381 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1441 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1501 ATCTTGTGGA AAGGACGACG GACGGCTCCC GCTGATGTTA GAACGCGTTA AGTCgacaat 1561 caacctctgg attacaaaat ttgtgaaaga tt