Construct: ORF TRCN0000476363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011863.1_s317c1
- Derived from:
- ccsbBroadEn_09860
- DNA Barcode:
- TTCCGTTAGGACTGTCTCCACGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF152 (220441)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220441 | RNF152 | ring finger protein 152 | NM_173557.3 | 99.8% | 100% | 180G>T |
| 2 | human | 220441 | RNF152 | ring finger protein 152 | XM_005266650.3 | 99.8% | 100% | 180G>T |
| 3 | human | 220441 | RNF152 | ring finger protein 152 | XM_005266652.3 | 99.8% | 100% | 180G>T |
| 4 | human | 220441 | RNF152 | ring finger protein 152 | XM_011525878.2 | 99.8% | 100% | 180G>T |
| 5 | human | 220441 | RNF152 | ring finger protein 152 | XM_011525879.2 | 99.8% | 100% | 180G>T |
| 6 | human | 220441 | RNF152 | ring finger protein 152 | XM_017025612.1 | 99.8% | 100% | 180G>T |
| 7 | human | 220441 | RNF152 | ring finger protein 152 | XM_017025613.1 | 99.8% | 100% | 180G>T |
| 8 | mouse | 320311 | Rnf152 | ring finger protein 152 | NM_001160368.1 | 88% | 97.5% | (many diffs) |
| 9 | mouse | 320311 | Rnf152 | ring finger protein 152 | NM_178779.4 | 88% | 97.5% | (many diffs) |
| 10 | mouse | 320311 | Rnf152 | ring finger protein 152 | XM_006529699.3 | 88% | 97.5% | (many diffs) |
| 11 | mouse | 320311 | Rnf152 | ring finger protein 152 | XM_017321207.1 | 88% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 678
- ORF length:
- 609
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagacgctg tcccaggact ctctgctgga atgtcagatc tgtttcaatt 121 actacagccc ccggcgcagg cccaagttgc tggactgcaa gcacacctgc tgttcagtgt 181 gcctgcagca gatgaggacc agccagaagg atgtgcggtg cccctggtgc cgcggtgtca 241 ccaagcttcc tcccggcttc tccgtgtcgc agctcccgga cgacccggag gtcctggctg 301 tcatcgccat tccacacact tccgaacaca ccccggtctt catcaaactt cccagcaatg 361 ggtgctacat gctgcccctg cccatctcca aggagcgtgc gctgctgccc ggagacatgg 421 gctgccgcct gctgcccggg agccagcaga agtccgtcac cgtggtgacc atccctgctg 481 aacagcagcc tctgcaaggt ggggctcccc aggaggcggt ggaggaggag caggacaggc 541 ggggcgtggt gaaaagctcc acctggtcGG GGGTGTGCAC TGTCATCTTG GTGGCTTGCG 601 TCTTGGTCTT CCTCCTCGGC ATCGTGCTTC ACAACATGTC TTGCATTTCT AAGCGCTTCA 661 CTGTGATATC CTGTGGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCCGTT AGGACTGTCT 841 CCACGACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt