Construct: ORF TRCN0000476429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001382.1_s317c1
- Derived from:
- ccsbBroadEn_04575
- DNA Barcode:
- AAGTAAGTGTTAATCTAGTACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM129 (92305)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92305 | TMEM129 | transmembrane protein 129 | NM_001127266.2 | 100% | 100% | |
| 2 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_005248038.4 | 79.8% | 79.8% | 0_1ins219 |
| 3 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_005248039.5 | 79.8% | 79.8% | 0_1ins219 |
| 4 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_011513594.3 | 79.8% | 79.8% | 0_1ins219 |
| 5 | human | 92305 | TMEM129 | transmembrane protein 129 | XM_017008834.1 | 79.8% | 79.8% | 0_1ins219 |
| 6 | human | 92305 | TMEM129 | transmembrane protein 129 | NM_138385.3 | 63.7% | 62.7% | (many diffs) |
| 7 | mouse | 68366 | Tmem129 | transmembrane protein 129 | NM_026698.2 | 84.4% | 89.2% | (many diffs) |
| 8 | mouse | 68366 | Tmem129 | transmembrane protein 129 | XM_006504080.1 | 67.4% | 70.7% | (many diffs) |
| 9 | mouse | 68366 | Tmem129 | transmembrane protein 129 | XM_006504081.1 | 67.4% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1155
- ORF length:
- 1086
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacagcccc gaggtgacct tcactctcgc ctatctggtg ttcgccgtgt 121 gcttcgtgtt cacgcccaac gagttccacg cggcggggct cacggtgcag aacctgctgt 181 cgggctggct gggcagcgag gacgccgcct tcgtgccctt ccacttgcgc cgcacggccg 241 ccacgctgtt gtgccactcg ctgctgccgc tcggctacta tgtgggcatg tgccttgcgg 301 cttcagaaaa gcggctccac gccctcagcc aggcccctga ggcctggcgg ctcttcctgc 361 tgctggccgt gaccctcccc tccatcgcct gcatcctgat ctactactgg tcccgtgacc 421 ggtgggcctg ccacccactg gcgcgcaccc tggccctcta cgccctccca cagtctggct 481 ggcaggctgt tgcctcctct gtcaacactg agttccggcg gattgacaag tttgccaccg 541 gtgcaccagg tgcccgtgtg attgtgacag acacgtgggt gatgaaggta accacctacc 601 gagtgcacgt ggcccagcag caggacgtgc acctgactgt gacggagtct cggcagcatg 661 agctctcgcc agactcgaac ttgcccgtgc agctcctcac catccgtgtg gccagcacca 721 accctgctgt gcaggccttt gacatctggc tgaactccac tgagtacggg gagctctgcg 781 agaagctccg ggcacccatc cgcagggcag cccatgtggT CATCCACCAG AGCCTGGGCG 841 ACCTGTTCCT GGAGACATTT GCCTCCCTGG TAGAGGTCAA CCCGGCCTAC TCAGTGCCCA 901 GCAGCCAGGA GCTGGAGGCC TGCATAGGCT GCATGCAGAC ACGTGCCAGC GTGAAGCTGG 961 TGAAGACCTG CCAGGAGGCA GCCACAGGCG AGTGCCAGCA GTGTTACTGC CGCCCCATGT 1021 GGTGCCTCAC CTGCATGGGC AAGTGGTTCG CCAGCCGCCA GGACCCCCTG CGCCCTGACA 1081 CCTGGCTGGC CAGCCGCGTG CCCTGCCCCA CCTGCCGCGC ACGCTTCTGC ATCCTGGATG 1141 TGTGCACCGT GCGCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAGTAAGTGT TAATCTAGTA 1321 CCCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt