Construct: ORF TRCN0000476437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016569.1_s317c1
- Derived from:
- ccsbBroadEn_06477
- DNA Barcode:
- CTCAGTTTATGAGATTATTAGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNJ5 (3762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3762 | KCNJ5 | potassium inwardly rectifyi... | NM_000890.5 | 99.6% | 99.7% | (many diffs) |
| 2 | human | 3762 | KCNJ5 | potassium inwardly rectifyi... | NM_001354169.2 | 99.6% | 99.7% | (many diffs) |
| 3 | human | 3762 | KCNJ5 | potassium inwardly rectifyi... | XM_011542809.2 | 99.6% | 99.7% | (many diffs) |
| 4 | human | 3762 | KCNJ5 | potassium inwardly rectifyi... | XM_011542810.3 | 99.6% | 99.7% | (many diffs) |
| 5 | mouse | 16521 | Kcnj5 | potassium inwardly-rectifyi... | NM_010605.4 | 88.7% | 94.5% | (many diffs) |
| 6 | mouse | 16521 | Kcnj5 | potassium inwardly-rectifyi... | XM_006510039.3 | 88.7% | 94.5% | (many diffs) |
| 7 | mouse | 16521 | Kcnj5 | potassium inwardly-rectifyi... | XM_017313155.1 | 88.7% | 94.5% | (many diffs) |
| 8 | mouse | 16521 | Kcnj5 | potassium inwardly-rectifyi... | XM_017313153.1 | 79.4% | 84.6% | (many diffs) |
| 9 | mouse | 16521 | Kcnj5 | potassium inwardly-rectifyi... | XM_017313154.1 | 79.4% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggcgattct aggaatgcca tgaaccagga catggagatt ggagtcactc 121 cctgggaccc caagaagatt ccaaaacagg cccgcgatta tgtccccatt gccacagacc 181 gtacgcgcct gctggccgag ggcaagaagc cacgccagcg ctacatggag aagagcggca 241 agtgcaacgt gcaccacggc aacgtccagg agacctaccg gtacctgagt gacctcttca 301 ccaccctggt ggacctcaag tggcgcttca acttgctcgt cttcaccatg gtttacactg 361 tcacctggct gttcttcggc ttcatttggt ggctcattgc ttatatccgg ggtgacctgg 421 accatgttgg cgaccaagag tggattcctt gtgttgaaaa cctcagtggc ttcgtgtccg 481 ctttcctgtt ctccattgag accgaaacaa ccattgggta tggcttccga gtcatcacag 541 agaagtgtcc agaggggatt atactcctct tggtccaggc catcctgggc tccatcgtca 601 atgccttcat ggtggggtgc atgtttgtca agatcagcca gcccaagaag agagcggaga 661 ccctcatgtt ttccaacaac gcagtcatct ccatgcggga cgagaagctg tgcctcatgt 721 tccgggtggg cgacctccgc aactcccaca tcgtggaggc ctccatccgg gccaagctca 781 tcaagtcccg gcagaccaaa gagggggagt tcatccccct gaaccagaca gacatcaacg 841 tgggctttga cacgggcgac gaccgcctct tcctggtgtc tcctctgatc atctcccacg 901 agatcaacga gaagagccct ttctgggaga tgtctcaggc TCAGCTGCAT CAGGAAGAGT 961 TTGAAGTTGT GGTCATTCTA GAAGGGATGG TGGAAGCCAC AGGCATGACC TGCCAAGCCC 1021 GGAGCTCCTA CATGGATACA GAGGTGCTCT GGGGCCACCG ATTCACACCA GTCCTCACCT 1081 TGGAAAAGGG CTTCTATGAG GTGGACTACA ACACCTTCCA TGATACCTAT GAGACCAACA 1141 CACCCAGCTG CTGTGCCAAG GAGCTGGCAG AAATGAAGAG GGAAGGCCGG CTCCTCCAGT 1201 ACCTCCCCAG CCCCCCACTG CTGGGGGGCT GTGCTGAGGC AGGGCTGGAT GCAGAGGCTG 1261 AGCAGAATGA AGAAGATGAG CCCAAGGGGC TGGGTGGGTC CAGGGAGGCC AGGGGCTCGG 1321 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACT CAGTTTATGA GATTATTAGA GCACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt