Construct: ORF TRCN0000476440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008164.1_s317c1
- Derived from:
- ccsbBroadEn_04894
- DNA Barcode:
- AGACTGGGTAGCTTTGGCAAGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOT12 (134526)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476440
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | NM_130767.3 | 100% | 100% | |
| 2 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_017009045.1 | 97.1% | 97.1% | (many diffs) |
| 3 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_006714531.2 | 94.9% | 94.9% | 1042_1043ins84 |
| 4 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_017009046.1 | 92.2% | 92.2% | (many diffs) |
| 5 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_017009047.1 | 91.2% | 91.1% | (many diffs) |
| 6 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_024454366.1 | 84.9% | 81.7% | (many diffs) |
| 7 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_006714532.3 | 67.9% | 67.7% | 1129G>A;1131_1131delCinsAAAAATATATACT;1134_1135ins519 |
| 8 | human | 134526 | ACOT12 | acyl-CoA thioesterase 12 | XM_017009048.1 | 61.1% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1734
- ORF length:
- 1665
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcggccg gcgcccggcg aggtggtcat gagccaagcc atccagccgg 121 cgcacgccac tgcgcgcggc gagctgagcg cggggcagct gctcaagtgg atcgacacca 181 ccgcctgcct ggcggctgag aaacatgctg gagtttcctg cgttacagcc tcagtggatg 241 acatacagtt tgaggagaca gctagagttg gacaagttat aaccatcaaa gcaaaagtta 301 ctagagcatt cagcacaagc atggagatca gtatcaaggt catggtacag gatatgctca 361 ctggcattga gaagcttgtt agtgtggctt tctccacatt tgtagccaaa ccagttggaa 421 aagaaaagat tcatttaaaa ccagtcacac ttctaactga acaagatcat gtggaacata 481 atctggctgc tgagagaagg aaagttcgat tacaacatga agataccttt aacaatttaa 541 tgaaggaaag tagcaaattt gatgatctca tttttgatga agaggaagga gcggtttcca 601 caaggggcac ctccgttcag agcattgaac tggtcctccc accccatgca aaccatcacg 661 gaaatacatt tggtggccag attatggcgt ggatggagac agtggctact atttctgcaa 721 gccgcctgtg ttgggctcat ccctttctga agtccgtaga tatgtttaag ttccggggac 781 catctacagt tggagatcgt cttgtcttca ctgccattgt caacaataca tttcagacct 841 gtgttgaagt tggagttcgc gtggaggcct ttgactgtca ggaatgggcc gagggccgag 901 ggcgtcacat caacagtgct tttctcattt acaatgctgc tgatgataag gaaaatctca 961 tcacgtttcc cagaatccaa cccatttcaa aggatgattt cagacgctat cggggagcta 1021 ttgcacgcaa gcgaattcgc ctaggcagaa aatatgttat ttcccacaaa gaagaggttc 1081 cactttgcat acactgggat atcagcaagc aggcatccct gagtgacagc aatgtggagg 1141 ccctcaaaaa actggcagcc aaaaggggtt gggaggttac cagcactgtg gaaaagataa 1201 aaatatatac tctggaagag catgatgttt tatctgtttg ggttgaaaag cacgtgggaa 1261 gtccagcaca tttggcttat cgtctcttgt ctgactttac aaagcgacct ttgtgggacc 1321 cccattttgt gtcctgtgaa gtcatagact gggtgagtga agatgatcag ctgtatcaca 1381 TCACCTGTCC TATACTGAAT GATGACAAAC CCAAAGACTT GGTAGTACTC GTATCACGAA 1441 GAAAACCCCT CAAAGATGGT AACACTTACA CAGTGGCAGT GAAGTCGGTC ATTTTGCCAT 1501 CGGTCCCCCC GTCTCCACAG TACATCAGAA GTGAAATCAT ATGTGCCGGA TTTCTCATCC 1561 ATGCTATTGA CAGCAATTCA TGCATCGTAT CTTACTTTAA CCATATGTCT GCTAGCATCC 1621 TTCCTTACTT TGCTGGAAAT CTTGGTGGCT GGTCAAAATC CATTGAAGAA ACAGCAGCCT 1681 CTTGTATACA GTTCTTAGAG AATCCTCCTG ATGATGGGTT TGTAAGCACA TTTTTGCCAA 1741 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1801 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1861 TATCTTGTGG AAAGGACGAA GACTGGGTAG CTTTGGCAAG TTGACGCGTT AAGTCgacaa 1921 tcaacctctg gattacaaaa tttgtgaaag att