Construct: ORF TRCN0000476440

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008164.1_s317c1
Derived from:
ccsbBroadEn_04894
DNA Barcode:
AGACTGGGTAGCTTTGGCAAGTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACOT12 (134526)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476440
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 134526 ACOT12 acyl-CoA thioesterase 12 NM_130767.3 100% 100%
2 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_017009045.1 97.1% 97.1% (many diffs)
3 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_006714531.2 94.9% 94.9% 1042_1043ins84
4 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_017009046.1 92.2% 92.2% (many diffs)
5 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_017009047.1 91.2% 91.1% (many diffs)
6 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_024454366.1 84.9% 81.7% (many diffs)
7 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_006714532.3 67.9% 67.7% 1129G>A;1131_1131delCinsAAAAATATATACT;1134_1135ins519
8 human 134526 ACOT12 acyl-CoA thioesterase 12 XM_017009048.1 61.1% 60.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1734
ORF length:
1665
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagcggccg gcgcccggcg aggtggtcat gagccaagcc atccagccgg
121cgcacgccac tgcgcgcggc gagctgagcg cggggcagct gctcaagtgg atcgacacca
181ccgcctgcct ggcggctgag aaacatgctg gagtttcctg cgttacagcc tcagtggatg
241acatacagtt tgaggagaca gctagagttg gacaagttat aaccatcaaa gcaaaagtta
301ctagagcatt cagcacaagc atggagatca gtatcaaggt catggtacag gatatgctca
361ctggcattga gaagcttgtt agtgtggctt tctccacatt tgtagccaaa ccagttggaa
421aagaaaagat tcatttaaaa ccagtcacac ttctaactga acaagatcat gtggaacata
481atctggctgc tgagagaagg aaagttcgat tacaacatga agataccttt aacaatttaa
541tgaaggaaag tagcaaattt gatgatctca tttttgatga agaggaagga gcggtttcca
601caaggggcac ctccgttcag agcattgaac tggtcctccc accccatgca aaccatcacg
661gaaatacatt tggtggccag attatggcgt ggatggagac agtggctact atttctgcaa
721gccgcctgtg ttgggctcat ccctttctga agtccgtaga tatgtttaag ttccggggac
781catctacagt tggagatcgt cttgtcttca ctgccattgt caacaataca tttcagacct
841gtgttgaagt tggagttcgc gtggaggcct ttgactgtca ggaatgggcc gagggccgag
901ggcgtcacat caacagtgct tttctcattt acaatgctgc tgatgataag gaaaatctca
961tcacgtttcc cagaatccaa cccatttcaa aggatgattt cagacgctat cggggagcta
1021ttgcacgcaa gcgaattcgc ctaggcagaa aatatgttat ttcccacaaa gaagaggttc
1081cactttgcat acactgggat atcagcaagc aggcatccct gagtgacagc aatgtggagg
1141ccctcaaaaa actggcagcc aaaaggggtt gggaggttac cagcactgtg gaaaagataa
1201aaatatatac tctggaagag catgatgttt tatctgtttg ggttgaaaag cacgtgggaa
1261gtccagcaca tttggcttat cgtctcttgt ctgactttac aaagcgacct ttgtgggacc
1321cccattttgt gtcctgtgaa gtcatagact gggtgagtga agatgatcag ctgtatcaca
1381TCACCTGTCC TATACTGAAT GATGACAAAC CCAAAGACTT GGTAGTACTC GTATCACGAA
1441GAAAACCCCT CAAAGATGGT AACACTTACA CAGTGGCAGT GAAGTCGGTC ATTTTGCCAT
1501CGGTCCCCCC GTCTCCACAG TACATCAGAA GTGAAATCAT ATGTGCCGGA TTTCTCATCC
1561ATGCTATTGA CAGCAATTCA TGCATCGTAT CTTACTTTAA CCATATGTCT GCTAGCATCC
1621TTCCTTACTT TGCTGGAAAT CTTGGTGGCT GGTCAAAATC CATTGAAGAA ACAGCAGCCT
1681CTTGTATACA GTTCTTAGAG AATCCTCCTG ATGATGGGTT TGTAAGCACA TTTTTGCCAA
1741CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1801ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1861TATCTTGTGG AAAGGACGAA GACTGGGTAG CTTTGGCAAG TTGACGCGTT AAGTCgacaa
1921tcaacctctg gattacaaaa tttgtgaaag att

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