Construct: ORF TRCN0000476455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011511.1_s317c1
- Derived from:
- ccsbBroadEn_11933
- DNA Barcode:
- GCGATAACAAACATGGATTTCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VILL (50853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50853 | VILL | villin like | NM_001370264.1 | 99.9% | 100% | 1872G>T |
| 2 | human | 50853 | VILL | villin like | NM_001370265.1 | 95.1% | 95.1% | 332_333ins99;1773G>T |
| 3 | human | 50853 | VILL | villin like | NM_015873.3 | 80.1% | 80.1% | 1_510del;2382G>T |
| 4 | human | 50853 | VILL | villin like | NR_163266.1 | 75.8% | (many diffs) | |
| 5 | human | 50853 | VILL | villin like | NR_163267.1 | 72.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2124
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gattcagtgg aatgggccca agaccagcat ttctgagaag gctcgggggc 121 tggctttgac ctacagcctc cgggacaggg aacgtggtgg tggtcgtgca cagattggtg 181 tggtggatga tgaggccaaa gccccggacc tcatgcagat catggaggct gtgctgggcc 241 gcagggtggg cagcctgcgt gccgccacgc ccagcaagga tatcaaccag ctgcagaagg 301 ccaatgttcg cctgtaccat gtctatgaga agggcaaaga cctggtggtc ctggagttgg 361 cgaccccccc actgacccag gacctgctgc aggaggagga cttctacatc ctggaccagg 421 gtggcttcaa gatctatgtg tggcagggac gcatgtctag cctccaggag agaaaggctg 481 ccttcagccg ggctgtgggc ttcatccagg ccaagggcta cccgacctac accaacgtgg 541 aggtggtgaa cgacggcgcc gagtcggccg cgttcaagca gctcttccgg acttggtctg 601 agaagcggcg caggaaccag aagctcggcg ggagggataa atcgattcat gtaaagctgg 661 acgtgggcaa gctgcacacc cagcctaagt tagcggccca gctcaggatg gtggacgacg 721 gctctgggaa ggtggaggtg tggtgcatcc aggacttaca caggcagccc gtggacccca 781 agcgtcatgg acagctgtgt gcaggcaact gctaccttgt gctctacaca taccagaggc 841 tgggccgtgt ccagtacatc ctgtacctat ggcagggcca ccaggccact gcggatgaga 901 ttgaggccct gaacagcaac gctgaggaac tagatgtcat gtatggtggc gtcctagtac 961 aggagcatgt gaccatgggc agcgagcccc cccacttcct cgccatcttc cagggccagc 1021 tggtgatctt ccaggagaga gctgggcacc atggaaaggg gcagtcagca tccaccacaa 1081 ggcttttcca agtgcaaggc actgacagcc acaacaccag gaccatggag gtgccagccc 1141 gtgcctcatc cctcaactcc agtgacatct tcttgctggt cacagccagc gtctgctacc 1201 tctggtttgg gaagggctgt aatggtgatc agcgtgagat ggcacgggtg gtggtcactg 1261 tcatttccag gaagaatgag gaaacggtgc tggagggtca ggagcctccc cacttctggg 1321 aggccctggg aggccgggcc ccctacccca gcaacaagag gctccctgag gaggtcccca 1381 gcttccagcc acgactgttt gagtgctcca gccacatggg ctgcctggtc ctcgcagaag 1441 tggggttctt cagccaggag gacctggaca agtatgacat catgttactg gacacctggc 1501 aggagatctt cctgtggctt ggggaagctg caagtgagtg gaaggaggcg gtggcctggg 1561 gccaggagta cctgaagact cacccagcag ggaggagccc ggccacaccc atcgtgctgg 1621 tcaagcaggg ccatgagcct cccaccttca ttggatggtt cttcacttgg gacccctaca 1681 agtggactag ccacccgtcc cacaaggaag tggtggatgg cagcccggca gcagcatcaa 1741 ccatctctga gataacagca gaagtcaaca acttgcggct atccagatgg ccgggcaatg 1801 gcagggcagg tgccgtggcc ctgcaggccc tcaagggctc ccaggacagc TCAGAGAATG 1861 ATCTGGTGCG AAGCCCCAAG TCGGCTGGCA GCAGAACCAG CAGCTCCGTC AGCAGCACCA 1921 GCGCCACGAT CAACGGTGGC CTGCGCCGGG AACAACTGAT GCACCAGGCT GTTGAGGACC 1981 TGCCAGAGGG CGTGGACCCT GCCCGCAGGG AGTTCTATCT CTCAGACTCT GACTTCCAAG 2041 ATATCTTTGG GAAATCCAAG GAGGAATTCT ACAGCATGGC CACGTGGAGG CAGCGGCAGG 2101 AGAAAAAGCA GCTGGGCTTC TTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2161 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2221 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CGATAACAAA 2281 CATGGATTTC CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2341 att