Construct: ORF TRCN0000476471

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010808.1_s317c1
Derived from:
ccsbBroadEn_04503
DNA Barcode:
ATCCAATTGGGACATCCTATATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UBE3D (90025)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476471
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 90025 UBE3D ubiquitin protein ligase E3D NM_198920.3 100% 100%
2 human 90025 UBE3D ubiquitin protein ligase E3D XM_011536241.3 98.7% 98.7% (many diffs)
3 human 90025 UBE3D ubiquitin protein ligase E3D XM_017011458.2 97.1% 96.2% (many diffs)
4 human 90025 UBE3D ubiquitin protein ligase E3D XM_017011459.2 96.8% 97.7% 1150_1151insTGGCCTTTTTGA;1154G>T;1156_1179del
5 human 90025 UBE3D ubiquitin protein ligase E3D XM_011536240.2 96.3% 96.7% 1151_1152delCAinsTGGCCTTTTT;1157_1158insT;1159_1191del
6 human 90025 UBE3D ubiquitin protein ligase E3D XM_011536239.3 95.9% 95% (many diffs)
7 human 90025 UBE3D ubiquitin protein ligase E3D NM_001304437.1 91.7% 91.7% 0_1ins96
8 human 90025 UBE3D ubiquitin protein ligase E3D NM_001350602.1 91.7% 91.7% 0_1ins96
9 human 90025 UBE3D ubiquitin protein ligase E3D XM_017011460.1 91.7% 91.7% 0_1ins96
10 human 90025 UBE3D ubiquitin protein ligase E3D XM_011536238.3 83.1% 81.8% 1149_1266del;1286_1404del
11 human 90025 UBE3D ubiquitin protein ligase E3D XR_002956314.1 61.1% (many diffs)
12 human 90025 UBE3D ubiquitin protein ligase E3D XR_001743730.2 60.6% 1_94del;760_864del;1367_1924del
13 human 90025 UBE3D ubiquitin protein ligase E3D XR_001743726.2 60.1% 1_94del;759_880del;1384_1941del
14 human 90025 UBE3D ubiquitin protein ligase E3D NR_146809.1 59.5% 1_123del;942_943ins26;1265_1891del
15 human 90025 UBE3D ubiquitin protein ligase E3D XR_002956315.1 58.4% 1_94del;830_1006del;1439_1996del
16 human 90025 UBE3D ubiquitin protein ligase E3D NR_146808.1 55.9% (many diffs)
17 human 90025 UBE3D ubiquitin protein ligase E3D NM_001350603.1 51.9% 51.9% 0_1ins561
18 human 90025 UBE3D ubiquitin protein ligase E3D NM_001350604.1 51.9% 51.9% 0_1ins561
19 human 90025 UBE3D ubiquitin protein ligase E3D XM_011536242.1 43.1% 41.8% 0_1ins561;588_705del;725_843del
20 human 90025 UBE3D ubiquitin protein ligase E3D NR_146807.2 37.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1236
ORF length:
1167
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggcttct gcggcggaga cgcgcgtgtt tctggaggtg cggggacagc
121tgcagagcgc gcttctgatc ctgggagaac cgaaagaagg aggtatgccc atgaatattt
181ccataatgcc atcttcactc cagatgaaaa cccctgaagg ctgcacagaa atccagcttc
241cagcagaggt caggcttgta ccttcctctt gccgtgggct acagtttgtt gttggagatg
301gactgcacct gcgactgcag acgcaagcaa aattaggcac aaaactgatt tcaatgttta
361atcaaagctc gcaaacccaa gaatgttgca cgttttattg ccaatcctgc ggtgaagtca
421taataaaaga caggaagctc ctcagggtgc tcccactgcc gagtgagaac tggggagctc
481tagttggaga atggtgttgt catcctgacc cctttgctaa taaatcactt catccgcaag
541agaatgactg ttttattgga gactctttct tcttggtgaa tttaagaacc agtttgtggc
601agcaaagacc tgaactatcc ccagtggaga tgtgctgtgt ttcttctgac aaccattgta
661aattggaacc aaaggcaaat accaaagtaa tttgtaagcg ttgcaaggta atgttgggag
721agaccgtgtc atcagaaacc accaagtttt atatgacaga gataattatt cagtcatctg
781agaggagttt tcctatcata ccaaggtctt ggtttgtcca gagcgtgatc gcccagtgtc
841tggtgcagct ctccTCTGCT AGAAGCACTT TTAGATTCAC GATTCAAGGT CAGGATGACA
901AAGTGTATAT CTTGCTATGG CTTTTAAATT CAGACAGTTT GGTGATTGAA TCTTTGAGAA
961ATTCCAAATA TATCAAAAAA TTCCCCTTGT TGGAAAACAC ATTCAAAGCC GATTCTAGTT
1021CTGCCTGGAG TGCTGTCAAG GTCCTCTACC AGCCATGCAT CAAAAGCAGG AATGAAAAAC
1081TTGTCAGCTT GTGGGAAAGT GACATCAGCG TCCACCCGCT AACCCTGCCC TCTGCAACCT
1141GCTTGGAGCT GCTGTTGATA TTGTCAAAGA GTAATGCCAA TCTGCCTTCA TCCCTTCGCC
1201GTGTGAATTC CTTTCAGGTG GCCTTTTTGA AGATGTTGCC AACTTTCTTG TACAAAGTGG
1261TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1321CTAGCCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1381AATCCAATTG GGACATCCTA TATAAACGCG TTAAGTCgac aatcaacctc tggattacaa
1441aatttgtgaa agatt

Download FASTA (ORF) (Full)