Construct: ORF TRCN0000476481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000851.1_s317c1
- Derived from:
- ccsbBroadEn_10376
- DNA Barcode:
- CTTCGTTGAAACCCAACGGCGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT14P1 (401135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000935.2 | 42.8% | 39.2% | (many diffs) |
| 2 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001256006.1 | 28% | 25.6% | (many diffs) |
| 3 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000932.2 | 27% | 24.7% | (many diffs) |
| 4 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000933.2 | 27% | 24.7% | (many diffs) |
| 5 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000934.2 | 27% | 24.7% | (many diffs) |
| 6 | human | 255928 | SYT14 | synaptotagmin 14 | NM_153262.4 | 26.1% | 23.9% | (many diffs) |
| 7 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001146262.3 | 25.2% | 23.1% | (many diffs) |
| 8 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001146264.3 | 24.1% | 22.1% | (many diffs) |
| 9 | human | 255928 | SYT14 | synaptotagmin 14 | NM_001146261.2 | 23.4% | 21.4% | (many diffs) |
| 10 | human | 401135 | SYT14P1 | synaptotagmin 14 pseudogene 1 | NR_027094.1 | 17.8% | 1_285del;754_2628del | |
| 11 | human | 255928 | SYT14 | synaptotagmin 14 | XM_017000931.1 | 17.1% | 15.7% | (many diffs) |
| 12 | human | 255928 | SYT14 | synaptotagmin 14 | XM_006711262.3 | 16.7% | 15.3% | (many diffs) |
| 13 | human | 255928 | SYT14 | synaptotagmin 14 | NR_027459.3 | 3.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 537
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttgtagt gaaagtacat ccgcatgtca gtctctcgaa gacgggtcag 121 ttccagaaat tctcattagc cTGCTTTATA ATGCCACAAA TGGGAGACTA TCAGCAGAAG 181 TGATAAAAGG CAGCCACTTA AAAAATTGGG CAGCAAACAG ACCACCCAAT ACATATGTTA 241 AGTTAACTCT ACGGAAATCC ACGGATCAAG AGATGTCCAA ATGCAAGATA TCCATCCGCA 301 GAGGGCGGCC AAATCCAGTA TACAAGGAAA CTTTTGTTTT CAAAGTGACC CTATTTCAGC 361 TTTCTCATGT GACACTCATG CTGTCTGTGT ATAACAAAAG CAGCATGAGA AGAAAGATGA 421 TAGGCTGGAT TTATTTAGGT CTCAACAGCT CTGGAGAAGA ACTCAATCAC TGGACTGAAA 481 TGAAAGAGTC AAAAGGACGG CAAGTACGTA GATGGCAGGC GTTGCTAGAG TCACGATTGC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACTTCGTTG AAACCCAACG GCGATAACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt