Transcript: Human NM_153262.4

Homo sapiens synaptotagmin 14 (SYT14), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
SYT14 (255928)
Length:
12063
CDS:
49..1716

Additional Resources:

NCBI RefSeq record:
NM_153262.4
NBCI Gene record:
SYT14 (255928)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_153262.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380545 GAAATTATGCAGTTCGGTTTA pLKO_005 1064 CDS 100% 10.800 15.120 N SYT14 n/a
2 TRCN0000350390 AGATGAAGCGCTGGGTAAATA pLKO_005 324 CDS 100% 15.000 10.500 N SYT14 n/a
3 TRCN0000382274 CACAGACATCCCAACATATAA pLKO_005 900 CDS 100% 15.000 10.500 N SYT14 n/a
4 TRCN0000002109 CCTTGTTCTTCTACCTATAAA pLKO.1 951 CDS 100% 15.000 10.500 N SYT14 n/a
5 TRCN0000381969 ACATGGCTCAGTTCCAGAAAT pLKO_005 1284 CDS 100% 13.200 9.240 N SYT14 n/a
6 TRCN0000093198 ACCTTGTTCTTCTACCTATAA pLKO.1 950 CDS 100% 13.200 9.240 N Syt14 n/a
7 TRCN0000314850 AGTTCTCCAACTCTATCATAA pLKO_005 2172 3UTR 100% 13.200 9.240 N SYT14 n/a
8 TRCN0000314848 CTTGTTCTTCTACCTATAAAG pLKO_005 952 CDS 100% 13.200 9.240 N SYT14 n/a
9 TRCN0000381956 TCTTTCAAGTGGCCCTATTTC pLKO_005 1511 CDS 100% 13.200 9.240 N SYT14 n/a
10 TRCN0000002105 AGTCCCATTAGAAGAGCTGTT pLKO.1 1787 3UTR 100% 4.050 2.835 N SYT14 n/a
11 TRCN0000002106 CGGTTTAGACTGTATGGTGTA pLKO.1 1078 CDS 100% 4.050 2.835 N SYT14 n/a
12 TRCN0000314849 GGCAGCCAAATCCAGTATATA pLKO_005 1478 CDS 100% 15.000 9.000 N SYT14 n/a
13 TRCN0000002108 CCTTATCCAGAACACACAATT pLKO.1 353 CDS 100% 13.200 7.920 N SYT14 n/a
14 TRCN0000002107 CCTGTGATATTGGAACCTTCT pLKO.1 1180 CDS 100% 4.050 2.430 N SYT14 n/a
15 TRCN0000314847 CCTGTGATATTGGAACCTTCT pLKO_005 1180 CDS 100% 4.050 2.430 N SYT14 n/a
16 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 6977 3UTR 100% 4.950 2.475 Y GJD4 n/a
17 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 6977 3UTR 100% 4.950 2.475 Y C9orf85 n/a
18 TRCN0000147682 GACTATCAGCAGAAGTGATAA pLKO.1 1340 CDS 100% 1.320 0.660 Y SYT14P1 n/a
19 TRCN0000147941 GAGATGTCCAAATGCAAGATA pLKO.1 1444 CDS 100% 5.625 2.813 Y SYT14P1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153262.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10630 pDONR223 100% 30.8% 26.4% None (many diffs) n/a
2 ccsbBroad304_10630 pLX_304 0% 30.8% 26.4% V5 (many diffs) n/a
3 TRCN0000481038 GTGACAGGGAACAATCAAACCCAA pLX_317 71% 30.8% 26.4% V5 (many diffs) n/a
4 ccsbBroadEn_10376 pDONR223 100% 26.1% 23.9% None (many diffs) n/a
5 ccsbBroad304_10376 pLX_304 0% 26.1% 23.9% V5 (many diffs) n/a
6 TRCN0000476481 CTTCGTTGAAACCCAACGGCGATA pLX_317 82.6% 26.1% 23.9% V5 (many diffs) n/a
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