Construct: ORF TRCN0000476499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005129.1_s317c1
- Derived from:
- ccsbBroadEn_08674
- DNA Barcode:
- ATAGAACACAATGGGTCCCTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDR39U1 (56948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476499
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56948 | SDR39U1 | short chain dehydrogenase/r... | NM_020195.2 | 99.5% | 98.9% | 234_235delAAinsTC;696G>C;809A>G |
| 2 | human | 56948 | SDR39U1 | short chain dehydrogenase/r... | XM_011536975.2 | 92.2% | 91.7% | (many diffs) |
| 3 | human | 56948 | SDR39U1 | short chain dehydrogenase/r... | NM_001290292.1 | 71.7% | 62.3% | (many diffs) |
| 4 | human | 56948 | SDR39U1 | short chain dehydrogenase/r... | NM_001308075.1 | 71.7% | 62.3% | (many diffs) |
| 5 | human | 56948 | SDR39U1 | short chain dehydrogenase/r... | NM_001290293.2 | 62.6% | 62.1% | (many diffs) |
| 6 | mouse | 654795 | Sdr39u1 | short chain dehydrogenase/r... | NM_001082975.1 | 85.4% | 84.6% | (many diffs) |
| 7 | mouse | 654795 | Sdr39u1 | short chain dehydrogenase/r... | XM_006519365.3 | 70.9% | 69.3% | (many diffs) |
| 8 | mouse | 654795 | Sdr39u1 | short chain dehydrogenase/r... | XM_006519366.2 | 55.5% | 54.7% | (many diffs) |
| 9 | mouse | 654795 | Sdr39u1 | short chain dehydrogenase/r... | XM_011245133.2 | 55.5% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 945
- ORF length:
- 879
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tgtgcttgtg ggtggcggga caggcttcat tgggacagcc ctaacccagc 121 tgctgaatgc cagaggccac gaagtgacgt tggtctcccg aaagcccggg cccggccgga 181 tcacgtggga tgagctcgct gcatcggggc tgccgagctg cgatgccgcc gtcaacctgg 241 ccggagagaa catcctcaac cctctccgaa gatggaatga aaccttccaa aaagaggttc 301 tcggcagccg cctagagacc acccaattgc tggctaaagc catcaccaaa gccccacaac 361 cccccaaggc ctgggtctta gtcacaggtg tagcttacta ccagcccagt ctgactgcgg 421 agtatgatga agacagccca ggaggggact ttgacttttt ctccaacctc gtaaccaaat 481 gggaagctgc agccaggctt cctggagatt ctacacgcca ggtggtggtg cgctcagggg 541 ttgtgctggg ccgtgggggt ggtgccatgg gccacatgct gctgcccttt cgcctgggcc 601 tggggggccc catcggctca ggccaccaat tcttcccctG GATACACATC GGGGACCTGG 661 CAGGAATCCT GACCCATGCC CTTGAAGCAA ACCACGTGCA CGGGGTCCTG AATGGAGTGG 721 CTCCATCCTC CGCCACTAAT GCTGAGTTTG CCCAGACCTT CGGTGCTGCC CTGGGCCGCC 781 GAGCCTTCAT CCCTCTCCCC AGCGCTGTGG TGCAAGCTGT CTTTGGGCGA CAGCGTGCCA 841 TCATGCTGCT GGAGGGCCAG AAGGTGATCC CACGGCGAAC ACTGGCCACT GGCTACCAGT 901 ATTCCTTCCC AGAGCTAGGG GCTGCCTTAA AGGAAATTGT AGCCTACCCA ACTTTCTTGT 961 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1021 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1081 GAAAGGACGA ATAGAACACA ATGGGTCCCT TACCACGCGT TAAGTCgaca atcaacctct 1141 ggattacaaa atttgtgaaa gatt