Construct: ORF TRCN0000476501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008001.1_s317c1
- Derived from:
- ccsbBroadEn_10185
- DNA Barcode:
- GTAGCGGCTTAACGCTTCAAGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERINC4 (619189)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476501
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 619189 | SERINC4 | serine incorporator 4 | NM_001258032.1 | 100% | 100% | |
| 2 | human | 619189 | SERINC4 | serine incorporator 4 | NM_001258031.1 | 52.8% | 46.2% | 1_520del;633_844del |
| 3 | mouse | 574418 | Serinc4 | serine incorporator 4 | XM_017319149.1 | 81.3% | 74.4% | (many diffs) |
| 4 | mouse | 574418 | Serinc4 | serine incorporator 4 | NM_001025371.2 | 43.4% | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 891
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcatctct tcccagcatg gcattacatt ggcatctgtg gaggctttgc 121 attcatccta ctgcagttgg tgcttattac agcttttgcc cattcctgga acaagaactg 181 agcaaccccg ctctggcctc ctacaagctt ctgtcatcag ctgctatatc atgtatctga 241 ctttctctgc actgtccagc cgtcctccag agagagtaat ccttcaagga cagaatcaca 301 ccctgtgcct gcctggcctg agtaaaatgg aaccccaaac accagatatc tctctagcaa 361 tgctgagtgc tagcatcatg tatgcttgtg tgctttttgc ttgcaatgag gcctcctacc 421 tggctgaggt atttggaccc ctgtggattg tcaaggttta cagctatgag tttcagaagc 481 cctcactgtg tttctgctgc ccTGAAACAG TGGAGGCAGA CAAAGGGCAA AGGGGTGGGG 541 CTGCGAGGCC AGCTGACCAA GAAACCCCTC CAGCTCCTCC AGTCCAAGTC CAGCATCTTT 601 CCTACAACTA TTCTGCCTTC CACTTCGTCT TCTTCCTTGC CTCACTCTAT GTCATGGTTA 661 CCCTTACCAA CTGGTTCAGC TATGAGGGAG CAGAACTGGA AAAGACCTTC ATCAAGGGTA 721 GCTGGGCCAC CTTCTGGGTC AAGGTTGCCT CATGCTGGGC CTGCGTACTC CTCTATCTGG 781 GGCTGTTACT GGCACCACTC TGTTGGCCCC CCACCCAGAA ACCCCAGCCC CTTATCTTGA 841 GGCGCCGCCG CCACCGCATC ATATCCCCAG ATAACAAATA TCCTCCAGTC TTGCCAACTT 901 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 961 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1021 CTTGTGGAAA GGACGAGTAG CGGCTTAACG CTTCAAGGTT ACGCGTTAAG TCgacaatca 1081 acctctggat tacaaaattt gtgaaagatt