Construct: ORF TRCN0000476502

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009722.1_s317c1
Derived from:
ccsbBroadEn_12318
DNA Barcode:
ACCCCCATCCAACTTCCGGATCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MFF (56947)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476502
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56947 MFF mitochondrial fission factor NM_001277064.2 100% 100%
2 human 56947 MFF mitochondrial fission factor XM_006712639.3 100% 100%
3 human 56947 MFF mitochondrial fission factor NM_001277068.1 94.4% 94.4% 441_482del
4 human 56947 MFF mitochondrial fission factor NM_001277063.2 91.9% 90.6% (many diffs)
5 human 56947 MFF mitochondrial fission factor XM_017004515.2 91.9% 90.6% (many diffs)
6 human 56947 MFF mitochondrial fission factor NM_001277065.2 91.5% 91.5% 440_441ins60
7 human 56947 MFF mitochondrial fission factor NM_001277066.2 91.5% 91.5% 440_441ins60
8 human 56947 MFF mitochondrial fission factor XM_005246687.4 90.1% 90.1% 1_78del
9 human 56947 MFF mitochondrial fission factor XM_017004513.2 86.3% 82.3% (many diffs)
10 human 56947 MFF mitochondrial fission factor XM_017004514.2 86.3% 82.3% (many diffs)
11 human 56947 MFF mitochondrial fission factor XM_005246686.4 83.2% 82% (many diffs)
12 human 56947 MFF mitochondrial fission factor XM_005246688.2 82.5% 82.5% 1_78del;518_519ins60
13 human 56947 MFF mitochondrial fission factor XM_005246685.4 82.3% 82.3% 1_78del;519_593del
14 human 56947 MFF mitochondrial fission factor XM_024453009.1 82.3% 82.3% 1_78del;519_593del
15 human 56947 MFF mitochondrial fission factor NM_001277062.2 81.7% 81.7% 441_599del
16 human 56947 MFF mitochondrial fission factor XM_006712638.2 81.7% 81.7% 441_599del
17 human 56947 MFF mitochondrial fission factor XM_024453008.1 81.7% 81.7% 441_599del
18 human 56947 MFF mitochondrial fission factor XM_005246684.2 78.8% 75.1% (many diffs)
19 human 56947 MFF mitochondrial fission factor XM_005246683.2 75.3% 75.3% 441_674del
20 human 56947 MFF mitochondrial fission factor XM_006712637.2 75.3% 75.3% 441_674del
21 human 56947 MFF mitochondrial fission factor XM_011511501.1 75.3% 75.3% 441_674del
22 human 56947 MFF mitochondrial fission factor XM_005246682.2 75% 75% 1_78del;519_677del
23 human 56947 MFF mitochondrial fission factor XM_017004512.1 75% 75% 1_78del;519_677del
24 human 56947 MFF mitochondrial fission factor XM_005246681.4 72.2% 69.5% (many diffs)
25 human 56947 MFF mitochondrial fission factor NM_001277061.2 69.5% 69.5% 1_78del;519_752del
26 human 56947 MFF mitochondrial fission factor NM_020194.5 69.5% 69.5% 1_78del;519_752del
27 human 56947 MFF mitochondrial fission factor XM_011511500.1 69.5% 69.5% 1_78del;519_752del
28 human 56947 MFF mitochondrial fission factor NM_001277067.1 67.9% 63.9% (many diffs)
29 human 644762 LOC644762 mitochondrial fission facto... NR_110534.1 37.3% (many diffs)
30 human 56947 MFF mitochondrial fission factor NR_102266.2 35.5% (many diffs)
31 mouse 75734 Mff mitochondrial fission factor NM_001310697.1 91% 94.1% (many diffs)
32 mouse 75734 Mff mitochondrial fission factor NM_001310695.1 90.6% 93.7% (many diffs)
33 mouse 75734 Mff mitochondrial fission factor NM_001372413.1 87% 89.9% (many diffs)
34 mouse 75734 Mff mitochondrial fission factor NM_001372412.1 86.6% 89.6% (many diffs)
35 mouse 75734 Mff mitochondrial fission factor NM_001310699.1 82.9% 85.7% (many diffs)
36 mouse 75734 Mff mitochondrial fission factor NM_001372414.1 82.5% 85.3% (many diffs)
37 mouse 75734 Mff mitochondrial fission factor XM_006496567.4 82% 84.8% (many diffs)
38 mouse 75734 Mff mitochondrial fission factor XM_006496566.4 81.7% 84.5% (many diffs)
39 mouse 75734 Mff mitochondrial fission factor XM_006496565.4 78.7% 81.4% (many diffs)
40 mouse 75734 Mff mitochondrial fission factor XM_006496564.3 78.5% 81.1% (many diffs)
41 mouse 75734 Mff mitochondrial fission factor NM_001372411.1 77.4% 77.2% (many diffs)
42 mouse 75734 Mff mitochondrial fission factor NM_001372409.1 77.1% 76.9% (many diffs)
43 mouse 75734 Mff mitochondrial fission factor XM_006496569.3 74.7% 77.2% (many diffs)
44 mouse 75734 Mff mitochondrial fission factor NM_029409.3 74.6% 76.9% (many diffs)
45 mouse 75734 Mff mitochondrial fission factor XM_006496568.4 74.4% 76.9% (many diffs)
46 mouse 75734 Mff mitochondrial fission factor NM_001372408.1 74.4% 76.7% (many diffs)
47 mouse 75734 Mff mitochondrial fission factor NM_001372407.1 71.9% 74.1% (many diffs)
48 mouse 75734 Mff mitochondrial fission factor NM_001372406.1 71.7% 73.9% (many diffs)
49 mouse 75734 Mff mitochondrial fission factor XM_006496563.4 70.7% 70.4% (many diffs)
50 mouse 75734 Mff mitochondrial fission factor XM_006496562.3 70.5% 70.2% (many diffs)
51 mouse 75734 Mff mitochondrial fission factor NM_001372405.1 68.5% 70.6% (many diffs)
52 mouse 75734 Mff mitochondrial fission factor XM_006496558.2 68.3% 70.4% (many diffs)
53 mouse 75734 Mff mitochondrial fission factor XM_006496557.4 66.2% 68.2% (many diffs)
54 mouse 75734 Mff mitochondrial fission factor XM_006496556.3 66% 68% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
780
ORF length:
714
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agaaattagt cgaattcagt acgaaatgga atatactgaa ggcattagtc
121agcgaatgag ggtcccagaa aagttaaaag tagcaccgcc aaacgctgac ctggaacaag
181gattccaaga aggagttcca aatgctagtg tgataatgca agttccggag aggattgttg
241tagcaggaaa taatgaagat gtttcatttt caagaccagc agatcttgac cttattcagt
301caactccctt taaacccctg gcactgaaaa caccacctcg tgtacttacg ctgagtgaaa
361gaccactaga ttttctggat ttagaaagac ctcctacaac ccctcaaaat gaagaaaTCC
421GAGCAGTTGG CAGACTAAAA AGAGAGCGGT CTATGAGTGA AAATGCTGTT CGCCAAAATG
481GACAGCTGGT CAGAAATGAT TCTCTACCTG TGTTGCGTGG TGGGTCTGCT GCCGCCACTT
541CTAATCCTCA TCATGACAAC GTCAGGTATG GCATTTCAAA TATAGATACA ACCATTGAAG
601GAACGTCAGA TGACCTGACT GTTGTAGATG CAGCTTCACT AAGACGACAG ATAATCAAAC
661TAAATAGACG TCTACAACTT CTGGAAGAGG AGAACAAAGA ACGTGCTAAA AGAGAAATGG
721TCATGTATTC AATTACTGTA GCTTTCTGGC TGCTTAATAG CTGGCTCTGG TTTCGCCGCT
781ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
841GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
901TTTATATATC TTGTGGAAAG GACGAACCCC CATCCAACTT CCGGATCTAA CGCGTTAAGT
961Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)