Construct: ORF TRCN0000476505
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015530.1_s317c1
- Derived from:
- ccsbBroadEn_01456
- DNA Barcode:
- ATGGACGCGGAGAATAGACAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS18 (6222)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476505
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6222 | RPS18 | ribosomal protein S18 | NM_022551.3 | 100% | 100% | |
| 2 | human | 645958 | RPS18P9 | ribosomal protein S18 pseud... | NR_077247.1 | 43.8% | (many diffs) | |
| 3 | mouse | 20084 | Rps18 | ribosomal protein S18 | NM_011296.2 | 91.8% | 100% | (many diffs) |
| 4 | mouse | 629591 | Gm6987 | predicted pseudogene 6987 | XM_894492.3 | 81.1% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 525
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctctagtg atccctgaaa agttccagca tattttgcga gtactcaaca 121 ccaacatcga tgggcggcgg aaaatagcct ttgccatcac tgccattaag ggtgtgggcc 181 GAAGATATGC TCATGTGGTG TTGAGGAAAG CAGACATTGA CCTCACCAAG AGGGCGGGAG 241 AACTCACTGA GGATGAGGTG GAACGTGTGA TCACCATTAT GCAGAATCCA CGCCAGTACA 301 AGATCCCAGA CTGGTTCTTG AACAGACAGA AGGATGTAAA GGATGGAAAA TACAGCCAGG 361 TCCTAGCCAA TGGTCTGGAC AACAAGCTCC GTGAAGACCT GGAGCGACTG AAGAAGATTC 421 GGGCCCATAG AGGGCTGCGT CACTTCTGGG GCCTTCGTGT CCGAGGCCAG CACACCAAGA 481 CCACTGGCCG CCGTGGCCGC ACCGTGGGTG TGTCCAAGAA GAAATTGCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA ATGGACGCGG AGAATAGACA ACCAACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt