Construct: ORF TRCN0000476506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010917.1_s317c1
- Derived from:
- ccsbBroadEn_09577
- DNA Barcode:
- CGCCTACTTGTCCCTATCCATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPANXN3 (139067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139067 | SPANXN3 | SPANX family member N3 | NM_001009609.4 | 99.5% | 99.2% | 21C>T;129C>G |
| 2 | human | 139067 | SPANXN3 | SPANX family member N3 | XM_017029264.1 | 84.9% | 81.9% | (many diffs) |
| 3 | human | 494119 | SPANXN2 | SPANX family member N2 | NM_001009615.2 | 69% | 60.7% | (many diffs) |
| 4 | human | 494118 | SPANXN1 | SPANX family member N1 | NM_001009614.3 | 43.9% | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 492
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacagcca acttccagta ccaatgggga gaagacgaag agcccctgtg 121 aatccaataa caaaaaaaat gatgagatgc aagaggtacc aaacagagtc ttagcccccg 181 aacagagttt gAAGAAGACA AAAACATCAG AATATCCAAT AATATTTGTG TATTACCTCA 241 GGAAGGGTAA GAAAATAAAT TCAAATCAAC TGGAGAATGA ACAGTCCCAA GAGAACTCCA 301 TCAATCCAAT CCAAAAGGAG GAGGACGAAG GCGTAGACTT ATCTGAAGGA TCTTCAAATG 361 AGGATGAAGA CCTAGGCCCA TGTGAAGGAC CTTCAAAGGA GGACAAAGAT CTAGACTCAT 421 CTGAAGGATC CTCACAGGAG GATGAAGACC TAGGCTTATC TGAAGGATCT TCACAGGACA 481 GTGGGGAGGA TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGC CTACTTGTCC CTATCCATCT 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t