Construct: ORF TRCN0000476513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001905.1_s317c1
- Derived from:
- ccsbBroadEn_15294
- DNA Barcode:
- TACTAGGCTGACTGCGAATCGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCLAT1 (253558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476513
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_182551.5 | 99.5% | 99.5% | 22_24delATTinsGGA;108_109delTGinsCA;1179C>T |
| 2 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_005264244.1 | 99.5% | 99.5% | 22_24delATTinsGGA;108_109delTGinsCA;1179C>T |
| 3 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_011532741.2 | 99.5% | 99.5% | 22_24delATTinsGGA;108_109delTGinsCA;1179C>T |
| 4 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_001002257.3 | 90.7% | 90.8% | 0_1ins114;1065C>T |
| 5 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_001304445.2 | 90.7% | 90.8% | 0_1ins114;1065C>T |
| 6 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_005264245.3 | 90.7% | 90.8% | 0_1ins114;1065C>T |
| 7 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_011532742.3 | 90.7% | 90.8% | 0_1ins114;1065C>T |
| 8 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_017003746.1 | 90.7% | 90.8% | 0_1ins114;1065C>T |
| 9 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | NM_001304446.2 | 60.7% | 60.8% | 0_1ins486;693C>T |
| 10 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_011532744.3 | 60.7% | 60.8% | 0_1ins486;693C>T |
| 11 | human | 253558 | LCLAT1 | lysocardiolipin acyltransfe... | XM_017003747.2 | 59.8% | 59.6% | (many diffs) |
| 12 | mouse | 225010 | Lclat1 | lysocardiolipin acyltransfe... | NM_001081071.2 | 77.1% | 80.9% | (many diffs) |
| 13 | mouse | 225010 | Lclat1 | lysocardiolipin acyltransfe... | NM_001177967.1 | 77.1% | 80.9% | (many diffs) |
| 14 | mouse | 225010 | Lclat1 | lysocardiolipin acyltransfe... | NM_001177968.1 | 77.1% | 80.9% | (many diffs) |
| 15 | mouse | 225010 | Lclat1 | lysocardiolipin acyltransfe... | XM_011246410.2 | 77.1% | 80.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1311
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcattccagg ggaagggaag gagtggtgct tctgaaccca tggtcaatta 121 acgaggcagt ttctagctac tgcacgtact tcataaagca ggactctaaa agcttcagaa 181 tcatggtgtc atggaaaggg atttacttta tactgactct gttttgggga agcttttttg 241 gaagcatttt catgctgagt ccctttttac ctttgatgtt tgtaaaccca tcttggtatc 301 gctggatcaa caaccgcctt gtggcaacat ggctcaccct acctgtggca ttattggaga 361 ccatgtttgg tgtaaaagtg attataactg gggatgcatt tgttcctgga gaaagaagtg 421 tcattatcat gaaccatcgg acaagaatgg actggatgtt cctgtggaat tgcctgatgc 481 gatatagcta cctcagattg gagaaaattt gcctcaaagc gagtctcaaa ggtgttcctg 541 gatttggttg ggccatgcag gctgctgcct atatcttcat tcataggaaa tggaaggatg 601 acaagagcca tttcgaagac atgattgatt acttttgtga tattcacgaa ccacttcaac 661 tcctcatatt cccagaaggg actgatctca cagaaaacag caagtctcga agtaatgcat 721 ttgctgaaaa aaatggactt cagaaatatg aatatgtttt acatccaaga actacaggct 781 ttacttttgt ggtagaccgt ctaagagaag gtaagaacct tgatgctgtc catgatatca 841 ctgtggcgta tcctcacaac attcctcaat cagagaagca cctcctccaa ggagactttc 901 ccagggaaat ccactttcac gtccaccggt atccaataga caccctcccc acatccaagg 961 aggaccTTCA ACTCTGGTGC CACAAACGGT GGGAAGAGAA AGAAGAGAGG CTGCGTTCCT 1021 TCTATCAAGG GGAGAAGAAT TTTTATTTTA CCGGACAGAG TGTCATTCCA CCTTGCAAGT 1081 CTGAACTCAG GGTCCTTGTG GTCAAATTGC TCTCTATACT GTATTGGACC CTGTTCAGCC 1141 CTGCAATGTG CCTACTCATA TATTTGTACA GTCTTGTTAA GTGGTATTTT ATAATCACCA 1201 TTGTAATCTT TGTGCTGCAA GAGAGAATAT TTGGTGGACT GGAGATTATA GAACTTGCAT 1261 GTTACCGACT TTTACACAAA CAGCCACATT TAAATTCAAA GAAAAATGAG TTGCCAACTT 1321 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1381 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1441 CTTGTGGAAA GGACGATACT AGGCTGACTG CGAATCGGAA ACGCGTTAAG TCgacaatca 1501 acctctggat tacaaaattt gtgaaagatt