Construct: ORF TRCN0000476522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005521.1_s317c1
- Derived from:
- ccsbBroadEn_08788
- DNA Barcode:
- CCCCTGTAATACTAGAGGTCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGR6 (59352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476522
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_021636.3 | 99.9% | 99.8% | 13A>G |
| 2 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_005245404.3 | 97.7% | 95.8% | (many diffs) |
| 3 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509838.2 | 95.1% | 93.2% | (many diffs) |
| 4 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509839.2 | 95.1% | 93.2% | (many diffs) |
| 5 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_001017403.1 | 93.6% | 92.9% | (many diffs) |
| 6 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001996.1 | 92.6% | 90.6% | (many diffs) |
| 7 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509841.2 | 90.8% | 90% | (many diffs) |
| 8 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001997.1 | 90.7% | 90.1% | (many diffs) |
| 9 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509840.2 | 90% | 88.1% | (many diffs) |
| 10 | human | 59352 | LGR6 | leucine rich repeat contain... | NM_001017404.1 | 87.4% | 85.6% | (many diffs) |
| 11 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509842.2 | 84.9% | 84.9% | 0_1ins414 |
| 12 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509843.2 | 84.9% | 84.9% | 0_1ins414 |
| 13 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509846.2 | 84.9% | 84.9% | 0_1ins414 |
| 14 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_011509844.2 | 77.2% | 77% | 1_1delAins619;4_5delCC;8_9insGAACT |
| 15 | human | 59352 | LGR6 | leucine rich repeat contain... | XM_017001998.1 | 66.3% | 64.9% | (many diffs) |
| 16 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_011248036.2 | 84.1% | 88.3% | (many diffs) |
| 17 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | NM_001033409.3 | 80.6% | 84.5% | (many diffs) |
| 18 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_006529714.1 | 78.3% | 82.2% | (many diffs) |
| 19 | mouse | 329252 | Lgr6 | leucine-rich repeat-contain... | XM_017321280.1 | 75% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2814
- ORF length:
- 2745
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaagacca gggttgacac ttgtttgtca agtgtcaata atcatctctg 121 cccgggacct cagcatgaac aacctcacag agcttcagcc tggcctcttc caccacctgc 181 gcttcttgga ggagctgcgt ctctctggga accatctctc acacatccca ggacaagcat 241 tctctggtct ctacagcctg aaaatcctga tgctgcagaa caatcagctg ggaggaatcc 301 ccgcagaggc gctgtgggag ctgccgagcc tgcagtcgct gcgcctagat gccaacctca 361 tctccctggt cccggagagg agctttgagg ggctgtcctc cctccgccac ctctggctgg 421 acgacaatgc actcacggag atccctgtca gggccctcaa caacctccct gccctgcagg 481 ccatgaccct ggccctcaac cgcatcagcc acatccccga ctacgcgttc cagaatctca 541 ccagccttgt ggtgctgcat ttgcataaca accgcatcca gcatctgggg acccacagct 601 tcgaggggct gcacaatctg gagacactag acctgaatta taacaagctg caggagttcc 661 ctgtggccat ccggaccctg ggcagactgc aggaactggg gttccataac aacaacatca 721 aggccatccc agaaaaggcc ttcatgggga accctctgct acagacgata cacttttatg 781 ataacccaat ccagtttgtg ggaagatcgg cattccagta cctgcctaaa ctccacacac 841 tatctctgaa tggtgccatg gacatccagg agtttccaga tctcaaaggc accaccagcc 901 tggagatcct gaccctgacc cgcgcaggca tccggctgct cccatcgggg atgtgccaac 961 agctgcccag gctccgagtc ctggaactgt ctcacaatca aattgaggag ctgcccagcc 1021 tgcacaggtg tcagaaattg gaggaaatcg gcctccaaca caaccgcatc tgggaaattg 1081 gagctgacac cttcagccag ctgagctccc tgcaagccct ggatcttagc tggaacgcca 1141 tccggtccat ccaccccgag gccttctcca ccctgcactc cctggtcaag ctggacctga 1201 cagacaacca gctgaccaca ctgcccctgg ctggacttgg gggcttgatg catctgaagc 1261 tcaaagggaa ccttgctctc tcccaggcct tctccaagga cagtttccca aaactgagga 1321 tcctggaggt gccttatgcc taccagtgct gtccctatgg gatgtgtgcc agcttcttca 1381 aggcctctgg gcagtgggag gctgaagacc ttcaccttga tgatgaggag tcttcaaaaa 1441 ggcccctggg cctccttgcc agacaagcag agaaccacta tgaccaggac ctggatgagc 1501 tccagctgga gatggaggac tcaaagccac accccagtgt ccagtgtagc cctactccag 1561 gccccttcaa gccctgtgag tacctctttg aaagctgggg catccgcctg gccgtgtggg 1621 ccatcgtgtt gctctccgtg ctctgcaatg gactggtgct gctgaccgtg ttcgctggcg 1681 ggcctgtccc cctgcccccg gtcaagtttg tggtaggtgc gattgcaggc gccaacacct 1741 tgactggcat ttcctgtggc cttctagcct cagtcgatgc cctgaccttt ggtcagttct 1801 ctgagtacgg agcccgctgg gagacggggc taggctgccg ggccactggc ttcctggcag 1861 tacttgggtc ggaggcatcg gtgctgctgc tcactctggc cgcagtgcag tgcagcgtct 1921 ccgtctcctg tgtccgggcc tatgggaagt ccccctccct gggcagcgtt cgagcagggg 1981 tcctaggctg cctggcactg gcagggctgg ccgccgcgct gcccctggcc tcagtgggag 2041 aatacggggc ctccccactc tgcctgccct acgcgccacc tgagggtcag ccagcagccc 2101 tgggcttcac cgtggccctg gtgatgatga actccttctg tttcctggtc gtggccggtg 2161 cctacatcaa actgtactgt gacctgccgc ggggcgactt tgaggccgtg tgggactgcg 2221 ccatggtgag gcacgtggcc tggctcatct tcgcagacgg gctcctctac tgtcccgtgg 2281 ccttcctcag ctttgcctcc atgctgggcc tcttccctgt cacgcccgag gccgtcaagt 2341 ctgtcctgct ggtggtgctg cccctgcctg cctgcctcaa cccactgctg tacctgctct 2401 tcaaccccca cttccgggat gaccttcggc ggcttcggcc ccgcgcaggg gactcagggc 2461 ccctagccta tgctgcggcc ggggagctgg agaagagctC CTGTGATTCT ACCCAGGCCC 2521 TGGTAGCCTT CTCTGATGTG GATCTCATTC TGGAAGCTTC TGAAGCTGGG CGGCCCCCTG 2581 GGCTGGAGAC CTATGGCTTC CCCTCAGTGA CCCTCATCTC CTGTCAGCAG CCAGGGGCCC 2641 CCAGGCTGGA GGGCAGCCAT TGTGTAGAGC CAGAGGGGAA CCACTTTGGG AACCCCCAAC 2701 CCTCCATGGA TGGAGAACTG CTGCTGAGGG CAGAGGGATC TACGCCAGCA GGTGGAGGCT 2761 TGTCAGGGGG TGGCGGCTTT CAGCCCTCTG GCTTGGCCTT TGCTTCACAC GTGTTGCCAA 2821 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2881 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2941 TATCTTGTGG AAAGGACGAC CCCTGTAATA CTAGAGGTCC ATAACGCGTT AAGTCgacaa 3001 tcaacctctg gattacaaaa tttgtgaaag att