Construct: ORF TRCN0000476569
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017894.1_s317c1
- Derived from:
- ccsbBroadEn_03245
- DNA Barcode:
- AACGGACTTGCCTCCATAAGCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLDN18 (51208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476569
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51208 | CLDN18 | claudin 18 | NM_016369.4 | 100% | 100% | |
| 2 | human | 51208 | CLDN18 | claudin 18 | NM_001002026.3 | 91.5% | 91.9% | (many diffs) |
| 3 | mouse | 56492 | Cldn18 | claudin 18 | NM_019815.3 | 86.3% | 87.8% | (many diffs) |
| 4 | mouse | 56492 | Cldn18 | claudin 18 | NM_001194922.1 | 67.5% | 70% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 852
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccaccacc acatgccaag tggtggcgtt cctcctgtcc atcctggggc 121 tggccggctg catcgcggcc accgggatgg acatgtggag cacccaggac ctgtacgaca 181 accccgtcac ctccgtgttc cagtacgaag ggctctggag gagctgcgtg aggcagagtt 241 caggcttcac cgaatgcagg ccctatttca ccatcctggg acttccagcc atgctgcagg 301 cagtgcgagc cctgatgatc gtaggcatcg tcctgggtgc cattggcctc ctggtatcca 361 tctttgccct gaaatgcatc cgcattggca gcatggagga ctctgccaaa gccaacatga 421 cactgaccTC CGGGATCATG TTCATTGTCT CAGGTCTTTG TGCAATTGCT GGAGTGTCTG 481 TGTTTGCCAA CATGCTGGTG ACTAACTTCT GGATGTCCAC AGCTAACATG TACACCGGCA 541 TGGGTGGGAT GGTGCAGACT GTTCAGACCA GGTACACATT TGGTGCGGCT CTGTTCGTGG 601 GCTGGGTCGC TGGAGGCCTC ACACTAATTG GGGGTGTGAT GATGTGCATC GCCTGCCGGG 661 GCCTGGCACC AGAAGAAACC AACTACAAAG CCGTTTCTTA TCATGCCTCA GGCCACAGTG 721 TTGCCTACAA GCCTGGAGGC TTCAAGGCCA GCACTGGCTT TGGGTCCAAC ACCAAAAACA 781 AGAAGATATA CGATGGAGGT GCCCGCACAG AGGACGAGGT ACAATCTTAT CCTTCCAAGC 841 ACGACTATGT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC GGACTTGCCT CCATAAGCTC 1021 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t