Construct: ORF TRCN0000476571
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000114.1_s317c1
- Derived from:
- ccsbBroadEn_10514
- DNA Barcode:
- GTGTTCCCCGCGGTCTGGCGAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TP73-AS1 (57212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476571
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57212 | TP73-AS1 | TP73 antisense RNA 1 | NR_033712.1 | 17.9% | (many diffs) | |
| 2 | human | 57212 | TP73-AS1 | TP73 antisense RNA 1 | NR_033708.1 | 14.7% | 1_1170del;1774_4077del | |
| 3 | human | 57212 | TP73-AS1 | TP73 antisense RNA 1 | NR_033709.1 | 12.8% | 1_1170del;1774_4690del | |
| 4 | human | 57212 | TP73-AS1 | TP73 antisense RNA 1 | NR_033710.1 | 10.1% | 1_1170del;1774_5945del | |
| 5 | human | 57212 | TP73-AS1 | TP73 antisense RNA 1 | NR_033711.1 | 9.4% | 1_1170del;1774_6409del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 672
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtcttttg tccagctcag ccgcctcgga tctcgctgcc accagcctta 121 ctgcacaccc gtgccacccg cccttgcccc gtcagcctca gcctcagctc attctctcag 181 gcagtcccag cattggcacg gtacctcctc ccgctgtggg ccacacgtct ctgctccctg 241 tcaaccctcc tgccatcagc accaccacca gcgacttgtc tgcccgggag gatgcaacac 301 catctgcctc caccggccac ctttcagtgt ttcctgcttt ccaagtaaag ataccagcag 361 tgccctcaga gcagaccagc cagagttttt ctgaagcctc ccacagggtg ctccccggag 421 gaggcccgag atgctctcgt gactttGGAG CCGGGGTGGC TGGCCACCTT GGCCTGGGCA 481 TCTTTGGGGT GGGCTTCGGG AGCCCGGCAC TGCTGCAGAG TGTGGTGGAT GAGAACAGCT 541 GCTGCTTGCT GTACGTGGTG GAGGACCAGC TGTGTGATGT GGAGCAAGCC TTCAGAGCTG 601 AGCATTTGGG CCAACACCAG AGTGGTTCGG GAACAGGATG CAGACATTGT CCTCAACGAC 661 CAGCGGCACT TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTG TTCCCCGCGG TCTGGCGAAC 841 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t