Construct: ORF TRCN0000476573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018232.1_s317c1
- Derived from:
- ccsbBroadEn_01405
- DNA Barcode:
- AGTCGATAAGCCTCGTACCGGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNASE2 (6036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6036 | RNASE2 | ribonuclease A family member 2 | NM_002934.3 | 100% | 100% | |
| 2 | human | 6037 | RNASE3 | ribonuclease A family member 3 | NM_002935.2 | 84.9% | 68.9% | (many diffs) |
| 3 | human | 643332 | ECRP | ribonuclease A family membe... | NR_033909.1 | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 552
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttccaaaa ctgttcactt cccaaatttg tctgcttctt ctgttggggc 121 ttctggctgt ggagggctca ctccatgtca aacctccaca gtttacctgg gctcaatggt 181 ttgaaaccca gcacatcaat atgacctccc agcaatgcac caatgcaatg caggtcatta 241 acaattatca acggcgatgc aaaaaccaaa atactttcct tcttacaact tttgctaacg 301 tagttaatgt ttgtggtaac ccaaatatga cctgtCCTAG TAACAAAACT CGCAAAAATT 361 GTCACCACAG TGGAAGCCAG GTGCCTTTAA TCCACTGTAA CCTCACAACT CCAAGTCCAC 421 AGAATATTTC AAACTGCAGG TATGCGCAGA CACCAGCAAA CATGTTCTAT ATAGTTGCAT 481 GTGACAACAG AGATCAACGA CGAGACCCTC CACAGTATCC GGTGGTTCCA GTTCACCTGG 541 ATAGAATCAT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGT CGATAAGCCT CGTACCGGTC 721 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t