Construct: ORF TRCN0000476580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013214.1_s317c1
- Derived from:
- ccsbBroadEn_09141
- DNA Barcode:
- ACACAAATACAAGTTTCCTGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM234A (83986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83986 | FAM234A | family with sequence simila... | NM_001284497.2 | 99.9% | 99.8% | 1649G>C |
| 2 | human | 83986 | FAM234A | family with sequence simila... | NM_032039.4 | 99.9% | 99.8% | 1649G>C |
| 3 | human | 83986 | FAM234A | family with sequence simila... | XM_005255622.1 | 99.9% | 99.8% | 1649G>C |
| 4 | human | 83986 | FAM234A | family with sequence simila... | XM_011522691.1 | 99.9% | 99.8% | 1649G>C |
| 5 | human | 83986 | FAM234A | family with sequence simila... | XM_011522692.1 | 99.9% | 99.8% | 1649G>C |
| 6 | human | 83986 | FAM234A | family with sequence simila... | XM_017023760.2 | 99.9% | 99.8% | 1649G>C |
| 7 | human | 83986 | FAM234A | family with sequence simila... | XM_017023761.1 | 99.9% | 99.8% | 1649G>C |
| 8 | human | 83986 | FAM234A | family with sequence simila... | XM_017023762.1 | 99.9% | 99.8% | 1649G>C |
| 9 | human | 83986 | FAM234A | family with sequence simila... | XM_005255623.3 | 98.3% | 98.1% | 383_384ins27;1622G>C |
| 10 | human | 83986 | FAM234A | family with sequence simila... | XM_006720957.1 | 98.3% | 98.1% | 813_814ins27;1622G>C |
| 11 | human | 83986 | FAM234A | family with sequence simila... | XM_017023763.2 | 98.3% | 98.1% | 383_384ins27;1622G>C |
| 12 | human | 83986 | FAM234A | family with sequence simila... | XM_017023764.1 | 98.3% | 98.1% | 383_384ins27;1622G>C |
| 13 | human | 83986 | FAM234A | family with sequence simila... | XM_017023765.2 | 96.6% | 96.5% | 383_384ins27;786_787ins27;1595G>C |
| 14 | human | 83986 | FAM234A | family with sequence simila... | NR_104317.1 | 61.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 taggcatgtt ggaccacaag gacttagagg ccgaaatcca ccccttgaaa aatgaagaaa 121 gaaaatcgca ggaaaatctg ggaaatccat caaaaaatga ggataacgtg aaaagcgcgc 181 ctccacagtc ccggctctcc cggtgccgag cggcggcgtt ttttctttca ttgtttctct 241 gcctttttgt ggtgttcgtc gtctcattcg tcatcccgtg tccagaccgg ccggcgtcac 301 agcgaatgtg gaggatagac tacagtgccg ctgttatcta tgactttctg gctgtggatg 361 atataaacgg ggacaggatc caagatgttc tttttcttta taaaaacacc aacagcagca 421 acaatttcag ccgatcctgt gtggacgaag gcttttcctc tccctgcacc tttgcagctg 481 ctgtgtcggg ggccaacggc agcacgctct gggagagacc tgtggcccaa gacgtggccc 541 tcgtggagtg tgctgtgccc cagccaagag gcagtgaggc accttctgcc tgcatcctgg 601 tgggcagacc cagttctttc attgcagtca acttgttcac aggggaaacc ctgtggaacc 661 acagcagcag cttcagcggg aatgcgtcca tcctgagccc tctgctgcag gtgcctgatg 721 tggacggcga tggggcccca gacctgctgg ttctcaccca ggagcgggag gaggttagtg 781 gccacctcta ctccggcagc accgggcacc agattggcct cagaggcagc cttggtgtgg 841 acggggaaag tggcttcctc cttcacgtca ccaggacagg tgcccactac atcctctttc 901 cctgcgcaag ctccctctgc ggctgctctg tgaagggtct ctacgagaag gtgaccggga 961 gcggcggccc gttcaagagt gacccgcact gggagagcat gctcaatgcc accacccgca 1021 ggatgctttc ccacagctct ggagcagtgc gctacctgat gcatgtccca gggaacgccg 1081 gtgcagatgt gcttcttgtg ggctcagagg ccttcgtgct gctggacggg caggagctga 1141 cgcctcgctg gacacccaag gcagcccatg tcctgagaaa acccatcttc ggccgctaca 1201 aaccagacac cttggctgta gccgttgaaa acggaactgg caccgacaga cagatcctgt 1261 ttctggacct tggcactgga gccgtcctgt gtagcctagc cctcccgagc ctccctgggg 1321 gtccactgtc cgccagcctg ccgaccgcag accaccgctc agccttcttc ttctggggcc 1381 tccacgagct ggggagcacc agcgagacgg agaccgggga ggcccggcac agcctgtaca 1441 tgttccaccc caccctgccg cgcgtgctgc tggagctggc caatgtctct acccacattg 1501 tcgcctttga cgccgtcctg tttgagcCAA GCCGCCACGC CGCCTACATC CTTCTGACAG 1561 GCCCGGCAGA CTCAGAGGCA CCCGGCCTGG TCTCTGTGAT CAAGCACAAG GTGCGGGACC 1621 TTGTCCCAAG CAGCAGGGTG GTCCGCCTGG GTGAGGGTGG GCCAGACAGT GACCAAGCCA 1681 TCAGGGACCG GTTCTCCCGG CTGCGGTACC AGACTGAGGC GTACCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGAACA CAAATACAAG TTTCCTGCAT GACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t