Construct: ORF TRCN0000476593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011294.1_s317c1
- Derived from:
- ccsbBroadEn_00010
- DNA Barcode:
- GGTCCCAGGTGTCCTGTTAGATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACO1 (48)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 48 | ACO1 | aconitase 1 | NM_001278352.1 | 100% | 100% | |
| 2 | human | 48 | ACO1 | aconitase 1 | NM_001362840.2 | 100% | 100% | |
| 3 | human | 48 | ACO1 | aconitase 1 | NM_002197.3 | 100% | 100% | |
| 4 | human | 48 | ACO1 | aconitase 1 | XM_011517888.2 | 100% | 100% | |
| 5 | mouse | 11428 | Aco1 | aconitase 1 | NM_007386.2 | 86.9% | 93.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2733
- ORF length:
- 2667
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caacccattc gcacaccttg ctgagccatt ggatcctgta caaccaggaa 121 agaaattctt caatttgaat aaattggagg attcaagata tgggcgctta ccattttcga 181 tcagagttct tctggaagca gccattcgga attgtgatga gtttttggtg aagaaacagg 241 atattgaaaa tattctacat tggaatgtca cgcagcacaa gaacatagaa gtgccattta 301 agcctgctcg tgtcatcctg caggacttta cgggtgtgcc cgctgtggtt gactttgctg 361 caatgcgtga tgctgtgaaa aagttaggag gagatccaga gaaaataaac cctgtctgcc 421 ctgctgatct tgtaatagat cattccatcc aggttgattt caacagaagg gcagacagtt 481 tacagaagaa tcaagacctg gaatttgaaa gaaatagaga gcgatttgaa tttttaaagt 541 ggggttccca ggcttttcac aacatgcgga ttattccccc tggctcagga atcatccacc 601 aggtgaattt ggaatatttg gcaagagtgg tatttgatca ggatggatat tattacccag 661 acagcctcgt gggcacagac tcgcacacta ccatgattga tggcttgggc attcttggtt 721 ggggtgtcgg tggtattgaa gcagaagctg tcatgctggg tcagccaatc agtatggtgc 781 ttcctcaggt gattggctac aggctgatgg ggaagcccca ccctctggta acatccactg 841 acatcgtgct caccattacc aagcacctcc gccaggttgg ggtagtgggc aaatttgtcg 901 agttcttcgg gcctggagta gcccagttgt ccattgctga ccgagctacg attgctaaca 961 tgtgtccaga gtacggagca actgctgcct ttttcccagt tgatgaagtt agtatcacgt 1021 acctggtgca aacaggtcgt gatgaagaaa aattaaagta tattaaaaaa tatcttcagg 1081 ctgtaggaat gtttcgagat ttcaatgacc cttctcaaga cccagacttc acccaggttg 1141 tggaattaga tttgaaaaca gtagtgcctt gctgtagtgg acccaaaagg cctcaggaca 1201 aagttgctgt gtccgacatg aaaaaggact ttgagagctg ccttggagcc aagcaaggat 1261 ttaaaggatt ccaagttgct cctgaacatc ataatgacca taagaccttt atctatgata 1321 acactgaatt cacccttgct catggttctg tggtcattgc tgccattact agctgcacaa 1381 acaccagtaa tccgtctgtg atgttagggg caggattgtt agcaaagaaa gctgtggatg 1441 ctggcctgaa cgtgatgcct tacatcaaaa ctagcctgtc tcctgggagt ggcgtggtca 1501 cctactacct acaagaaagc ggagtcatgc cttatctgtc tcagcttggg tttgacgtgg 1561 tgggctatgg ctgcatgacc tgcattggca acagtgggcc tttacctgaa cctgtggtag 1621 aagccatcac acagggagac cttgtagctg ttggagtact atctggaaac aggaattttg 1681 aaggtcgagt tcaccccaac acccgggcca actatttagc ctctcccccc ttagtaatag 1741 catatgcaat tgctggaacc atcagaatcg actttgagaa agagccattg ggagtaaatg 1801 caaagggaca gcaggtattt ctgaaagata tctggccgac tagagacgag atccaggcag 1861 tggagcgtca gtatgtcatc ccggggatgt ttaaggaagt ctatcagaaa atagagactg 1921 tgaatgaaag ctggaatgcc ttagcaaccc catcagataa gctgtttttc tggaattcca 1981 aatctacgta tatcaaatca ccaccattct ttgaaaacct gactttggat cttcagcccc 2041 ctaaatctat agtggatgcc tatgtgctgc taaatttggg agattcggta acaactgacc 2101 acatctcccc agctggaaat attgcaagaa acagtcctgc tgctcgctac ttaactaaca 2161 gaggcctaac tccacgagaa ttcaactcct atggctcccg ccgaggtaat gacgccgtca 2221 tggcacgggg aacatttgcc aacattcgct tgttaaacag atttttgaac aagcaggcac 2281 cacagactat ccatctgcct tctggggaaa tccttgatgt gtttgatgct gctgagcggt 2341 accagcaggc aggccTTCCC CTGATCGTTC TGGCTGGCAA AGAGTACGGT GCAGGCAGCT 2401 CCCGAGACTG GGCAGCTAAG GGCCCTTTCC TGCTGGGAAT CAAAGCCGTC CTGGCCGAGA 2461 GCTACGAGCG CATTCACCGC AGTAACCTGG TTGGGATGGG TGTGATCCCA CTTGAATATC 2521 TCCCTGGTGA GAATGCAGAT GCCCTGGGGC TCACAGGGCA AGAACGATAC ACTATCATTA 2581 TTCCAGAAAA CCTCAAACCA CAAATGAAAG TCCAGGTCAA GCTGGATACT GGCAAGACCT 2641 TCCAGGCTGT CATGAGGTTT GACACTGATG TGGAGCTCAC TTATTTCCTC AACGGGGGCA 2701 TCCTCAACTA CATGATCCGC AAGATGGCCA AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 2761 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2821 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 2881 TCCCAGGTGT CCTGTTAGAT TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2941 ttgtgaaaga tt