Construct: ORF TRCN0000476594

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000558.1_s317c1
Derived from:
ccsbBroadEn_07960
DNA Barcode:
GTTACCTGTAGACCGCGAGGTCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF19A (25897)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476594
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25897 RNF19A ring finger protein 19A, RB... NM_001280539.2 99.9% 99.7% 889G>T;1435G>T
2 human 25897 RNF19A ring finger protein 19A, RB... NM_001353837.1 99.9% 99.7% 889G>T;1435G>T
3 human 25897 RNF19A ring finger protein 19A, RB... NM_001353838.2 99.9% 99.7% 889G>T;1435G>T
4 human 25897 RNF19A ring finger protein 19A, RB... NM_015435.4 99.9% 99.7% 889G>T;1435G>T
5 human 25897 RNF19A ring finger protein 19A, RB... NM_183419.4 99.9% 99.7% 889G>T;1435G>T
6 human 25897 RNF19A ring finger protein 19A, RB... XM_017013302.1 99.9% 99.7% 889G>T;1435G>T
7 human 25897 RNF19A ring finger protein 19A, RB... XM_017013303.2 99.9% 99.7% 889G>T;1435G>T
8 human 25897 RNF19A ring finger protein 19A, RB... XM_024447118.1 99.9% 99.7% 889G>T;1435G>T
9 human 25897 RNF19A ring finger protein 19A, RB... XM_024447119.1 99.9% 99.7% 889G>T;1435G>T
10 human 25897 RNF19A ring finger protein 19A, RB... XM_011516956.3 93.5% 93.4% 889G>T;1300_1301ins162
11 human 25897 RNF19A ring finger protein 19A, RB... XM_017013304.1 93.5% 93.4% 889G>T;1300_1301ins162
12 human 25897 RNF19A ring finger protein 19A, RB... XM_024447120.1 62.9% 62.8% 0_1ins930;505G>T
13 human 25897 RNF19A ring finger protein 19A, RB... XR_928315.3 53% (many diffs)
14 mouse 30945 Rnf19a ring finger protein 19A NM_013923.2 88.4% 93.3% (many diffs)
15 mouse 30945 Rnf19a ring finger protein 19A XM_006520081.3 59.9% 56.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2583
ORF length:
2514
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcaagaacaa gaaataggtt ttatctctaa atataatgaa gggctgtgtg
121taaacactga ccctgtctca attctaacaa gcattttaga catgagttta catcggcaaa
181tgggttcaga tcgagatctt cagtcctctg cttcatctgt gagcttgcct tcagtcaaaa
241aggcacccaa aaaaagaaga atttcaatag gctccctgtt tcggaggaaa aaagataaca
301aacgtaaatc aagggagcta aatggcgggg tggatggaat tgcaagtatt gaaagtatac
361attctgaaat gtgtactgat aagaactcca ttttctctac aaatacctct tctgacaatg
421gattaacttc catcagcaaa caaattggag acttcataga gtgccctttg tgccttttgc
481ggcattctaa agacagattt cctgatataa tgacttgtca tcacagatct tgtgtggatt
541gcttacgaca atatttaagg atagaaatct ctgaaagcag agttaatatt agttgcccag
601aatgtactga acggtttaat ccccatgata ttcgcttgat attaagtgat gatgtcttga
661tggaaaaata cgaagaattt atgcttagac ggtggcttgt tgcagatcct gattgtaggt
721ggtgtccagc tccagactgt ggatatgctg tgatagcatt tggatgtgcc agctgtccaa
781aattaacttg tgggcgagag ggctgtggaa cagagttttg ctaccactgt aaacagattt
841ggcaccccaa ccagacctgt gatgctgctc gacaagagag agcccagagc ttacgtttga
901gaactatacg ttcttcatcc attagttata gtcaagagtc tggagcagca gctgattata
961taaagccatg tccacgatgt gctgcttata taataaagat gaatgatggg agctgcaatc
1021acatgacatg tgctgtttgt ggttgtgagt tttgttggtt gtgtatgaaa gaaatctcag
1081atttgcatta tctaagtcca tcaggatgta ctttttgggg gaagaaaccc tggagccgaa
1141agaagaaaat attgtggcaa ctgggaacac tggttggtgc tcctgtcgga atcgctttaa
1201tagctggcat tgctattcct gcaatgatta ttggcattcc tgtgtatgtg ggccgcaaga
1261ttcacaatcg ctatgaaggc aaggatgttt caaagcacaa acggaatttg gccatagcag
1321gtggtgtaac gttgtctgta atcgtgtctc cagtagtagc tgcagtgact gtaggtatcg
1381gtgttcctat tatgttagct tatgtctatg gcgtagttcc aatttctctt tgtcgaagcg
1441gaggttgtgg agtctcagca ggcaatggaa aaggagttag gattgaattt gatgatgaaa
1501attatataaa tgttggtgga actaacacag ctgtagacac aacatcagta gcagaagcaa
1561gacacaaccc aagcataggg gagggaagtg ttggtgggct gactggcagt ttgagtgcaa
1621gtggaagcca catggatcga ataggagcca tccgagacaa cctgagtgaa acggccagca
1681ccatggcact agctggagcc agtataacgg ggagtctgtc aggaagtgcc atggtaaact
1741gttttaacag gttggaagta caagcagatg tacagaaaga acggtacagt ctaagtggag
1801aatctggcac agtcagcttg ggaacagtta gtgataatgc cagcaccaaa gcaatggcag
1861gatccattct gaattcctac atcccattgg acaaagaagg caacagtatg gaggtgcaag
1921tagatattga gtcaaagcca tccaaattca ggcacaacag tggaagcagt agtgtggatg
1981atggcagtgc cacccgaagt catgctggcg gttcatccag tggcttgcct gaaggtaaat
2041ctagtgccac caagtggtcc aaagaagcaa cagcagggaa aaaatcaaaa agtggtaaac
2101tgaggaaaaa gggtaacatg aagataaatg agacgagaga ggacatggat gcacagttgt
2161tagaacaaca aagcacgaac tcaagtgaat ttgaggctcc atccctcagt gacagtatgc
2221cttctgtagc agattctcac tctagtcatt tttctgaatt tagttgttct gacctagaaa
2281gcatgaaaac ttcttGTAGT CATGGTTCCA GTGATTATCA CACCCGCTTT GCTACTGTTA
2341ACATTCTTCC TGAGGTAGAA AATGACCGTC TGGAAAATTC CCCACATCAG TGTAGCATTT
2401CTGTGGTTAC CCAAACTGCT TCCTGTTCAG AAGTTTCACA GTTGAATCAT ATTGCTGAAG
2461AACATGGTAA CAATGGAATA AAACCTAATG TTGATTTATA TTTTGGCGAT GCACTAAAAG
2521AAACAAATAA CAACCACTCA CATCAGACAA TGGAATTAAA AGTTGCAATT CAGACTGAAA
2581TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2641TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2701GGCTTTATAT ATCTTGTGGA AAGGACGAGT TACCTGTAGA CCGCGAGGTC CCACGCGTTA
2761AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)