Construct: ORF TRCN0000476651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001783.1_s317c1
- Derived from:
- ccsbBroadEn_12411
- DNA Barcode:
- GATGCCGCAGTATTATAAACGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEUROD4 (58158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58158 | NEUROD4 | neuronal differentiation 4 | NM_021191.3 | 77% | 77% | 1_228del |
| 2 | mouse | 11923 | Neurod4 | neurogenic differentiation 4 | NM_001329489.1 | 67.3% | 70.3% | (many diffs) |
| 3 | mouse | 11923 | Neurod4 | neurogenic differentiation 4 | NM_001329490.1 | 67.3% | 70.3% | (many diffs) |
| 4 | mouse | 11923 | Neurod4 | neurogenic differentiation 4 | NM_007501.5 | 67.3% | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 834
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccaaagct cgccttgaga gattcagggc tcgaagagtc aaggctaatg 121 ccagagaacg gacccggatg catggcctga atgacgccct ggataacctg aggcgagtca 181 tgccatgcta ctctaaaacc caaaaacttt ccaagataga gactcttaga ctggccagga 241 actatatttg ggctttatct gaagtcctgg agactggcca gacacctgaa gggaaaggct 301 ttgtggagat gctgtgtaaa gggctctctc agcccacaag caacctggtg gctggatgtc 361 tccaactggg ccctcagtct gtcctcctgg agaagcacga ggataaatct cctatttgtg 421 actctgccat ctctgtccac aacttcaact atcagtctcc ggggcttcct agccctcctt 481 atggtcatat ggaaacacat ctccTTCATC TCAAGCCCCA AGTATTCAAG AGTTTGGGAG 541 AATCGTCCTT TGGGAGCCAT CTGCCTGACT GCAGTACACC CCCTTATGAG GGCCCACTCA 601 CTCCACCCCT GAGCATCAGT GGGAACTTCT CCTTGAAGCA AGATGGGTCT CCTGACCTAG 661 AAAAATCCTA CAGCTTCATG CCACATTACC CTTCTTCAAG TCTAAGCTCA GGGCATGTGC 721 ATTCAACTCC TTTTCAGGCT GGTACCCCCC GTTATGATGT TCCTATAGAC ATGTCCTATG 781 ATTCCTACCC CCATCATGGT ATTGGGACCC AACTCAATAC AGTCTTCACT GAGTTGCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAG ATGCCGCAGT ATTATAAACG GGTACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att