Construct: ORF TRCN0000476654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016721.1_s317c1
- Derived from:
- ccsbBroadEn_11922
- DNA Barcode:
- GGACAGAGAGTCTGTGATTTCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLK12 (43849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_001370126.1 | 100% | 100% | |
| 2 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_001370127.1 | 90.2% | 81.2% | (many diffs) |
| 3 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_001370128.1 | 90.2% | 81.2% | (many diffs) |
| 4 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_145895.2 | 90.2% | 81.2% | (many diffs) |
| 5 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_019598.3 | 56.6% | 39.8% | 1_70del;198_457del |
| 6 | human | 43849 | KLK12 | kallikrein related peptidas... | XM_005258951.3 | 52.3% | 11.7% | 1_70del;333_334ins169 |
| 7 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_001370125.1 | 51.6% | 32% | (many diffs) |
| 8 | human | 43849 | KLK12 | kallikrein related peptidas... | NM_145894.2 | 51.6% | 32% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 501
- ORF length:
- 432
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcactgagt gtgggcgtaa ctcacagccg tggcaggtgg ggctgtttga 121 gggcaccagc ctgcgctgcg ggggtgtcct tattgaccac aggtgggtcc tcacagcggc 181 tcactgcagc ggcagaccca ttcccggatc tgctccagtg cctcaacctc TCCATCGTCT 241 CCCATGCCAC CTGCCATGGT GTGTATCCCG GGAGAATCAC GAGCAACATG GTGTGTGCAG 301 GCGGCGTCCC GGGGCAGGAT GCCTGCCAGG GTGATTCTGG GGGCCCCCTG GTGTGTGGGG 361 GAGTCCTTCA AGGTCTGGTG TCCTGGGGGT CTGTGGGGCC CTGTGGACAA GATGGCATCC 421 CTGGAGTCTA CACCTATATT TGCAACTCCA CTCTTGTTGG CCTGGGAACT TCTTGGAACT 481 TTAACTCCTG CCAGCCCTTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAC AGAGAGTCTG 661 TGATTTCTCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt