Transcript: Human NM_145895.2

Homo sapiens kallikrein related peptidase 12 (KLK12), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
KLK12 (43849)
Length:
922
CDS:
298..714

Additional Resources:

NCBI RefSeq record:
NM_145895.2
NBCI Gene record:
KLK12 (43849)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_145895.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000372696 GGGTGTCCTTATTGACCACAG pLKO_005 371 CDS 100% 2.250 3.150 N KLK12 n/a
2 TRCN0000049925 AGGCAGCCACACCGAAGATTT pLKO.1 274 5UTR 100% 13.200 9.240 N KLK12 n/a
3 TRCN0000049923 CGGGAGAATCACGAGCAACAT pLKO.1 498 CDS 100% 4.950 3.465 N KLK12 n/a
4 TRCN0000372695 GAGTCCTTCAAGGTCTGGTGT pLKO_005 590 CDS 100% 2.640 1.848 N KLK12 n/a
5 TRCN0000049927 GCATCCCTGGAGTCTACACCT pLKO.1 644 CDS 100% 0.880 0.616 N KLK12 n/a
6 TRCN0000049924 CCAGTGCCTCAACCTCTCCAT pLKO.1 444 CDS 100% 0.880 0.528 N KLK12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145895.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11922 pDONR223 100% 90.2% 81.2% None (many diffs) n/a
2 ccsbBroad304_11922 pLX_304 0% 90.2% 81.2% V5 (many diffs) n/a
3 TRCN0000476654 GGACAGAGAGTCTGTGATTTCTCC pLX_317 61.7% 90.2% 81.2% V5 (many diffs) n/a
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