Construct: ORF TRCN0000476664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006549.1_s317c1
- Derived from:
- ccsbBroadEn_09392
- DNA Barcode:
- AGAGCCGGGTCTCATCACCTAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BUD23 (114049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NM_017528.5 | 99.8% | 100% | 348T>C |
| 2 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NM_001202560.3 | 94.1% | 94.2% | 348T>C;699_749del |
| 3 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XM_011515779.2 | 88.1% | 73.1% | 348T>C;638_639ins59;744_745ins40 |
| 4 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XM_006715847.1 | 86.5% | 79.2% | 0_1ins28;59_155del;417T>C |
| 5 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XM_011515778.1 | 76% | 56.7% | (many diffs) |
| 6 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NR_045512.2 | 62% | (many diffs) | |
| 7 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | NR_037776.3 | 60.2% | (many diffs) | |
| 8 | human | 114049 | BUD23 | BUD23 rRNA methyltransferas... | XR_002956407.1 | 16.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 909
- ORF length:
- 843
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcccgcggc cggcgtccgg agcatggcgg acccccagag ctgttttatg 121 acgagacaga agcccggaaa tacgttcgca actcacggat gattgatatc cagaccagga 181 tggctgggcg agcattggag cttctttatc tgccagagaa taagccctgt tacctgctgg 241 atattggctg tggcactggg ctgagtggaa gttatctgtc agatgaaggg cactattggg 301 tgggcctgga tatcagccct gccatgctgg atgaggctgt ggaccgagag atagagggag 361 acctgctgct gggggatatg ggccagggca tcccattcaa gccaggcaca ttcgatggtt 421 gcatcagcat ttctgctgtg cagtggctct gtaatgctaa caagaagtct gaaaaccctg 481 ccaagcgcct gtactgcttt tttgcttctc ttTTTTCTGT TCTCGTCCGG GGATCCCGAG 541 CTGTCCTGCA GCTGTACCCT GAGAACTCAG AGCAGTTGGA GCTGATCACA ACCCAGGCCA 601 CAAAGGCAGG CTTCTCCGGT GGCATGGTGG TAGACTACCC TAACAGTGCC AAAGCAAAGA 661 AATTCTACCT CTGCTTGTTT TCTGGGCCTT CGACCTTTAT ACCAGAGGGG CTGAGTGAAA 721 ATCAGGATGA AGTTGAACCC AGGGAGTCTG TGTTCACCAA TGAGAGGTTC CCATTAAGGA 781 TGTCGAGGCG GGGAATGGTG AGGAAGAGTC GGGCATGGGT GCTGGAGAAG AAGGAGCGGC 841 ACAGGCGCCA GGGCAGGGAA GTCAGACCTG ACACCCAGTA CACCGGCCGC AAGCGCAAGC 901 CCCGCTTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 961 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1021 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGAGCC GGGTCTCATC ACCTAGGAAC 1081 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt