Construct: ORF TRCN0000476678
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004642.1_s317c1
- Derived from:
- ccsbBroadEn_07626
- DNA Barcode:
- CTCTAATCCCCGTCTACTAGGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBLN5 (10516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476678
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10516 | FBLN5 | fibulin 5 | NM_006329.3 | 99.9% | 100% | 945T>C |
| 2 | human | 10516 | FBLN5 | fibulin 5 | XM_005267267.3 | 95.9% | 94.2% | (many diffs) |
| 3 | human | 10516 | FBLN5 | fibulin 5 | XM_011536357.2 | 88.8% | 85.5% | (many diffs) |
| 4 | human | 10516 | FBLN5 | fibulin 5 | XM_017020929.2 | 87.4% | 87.5% | 0_1ins168;777T>C |
| 5 | human | 10516 | FBLN5 | fibulin 5 | XM_011536356.1 | 85.4% | 80.7% | (many diffs) |
| 6 | human | 10516 | FBLN5 | fibulin 5 | XM_011536358.2 | 77.1% | 73.7% | (many diffs) |
| 7 | mouse | 23876 | Fbln5 | fibulin 5 | NM_011812.4 | 89.4% | 94.1% | (many diffs) |
| 8 | mouse | 23876 | Fbln5 | fibulin 5 | XM_006515866.1 | 89.3% | 94.1% | (many diffs) |
| 9 | mouse | 23876 | Fbln5 | fibulin 5 | XM_006515864.1 | 86.7% | 90.8% | (many diffs) |
| 10 | mouse | 23876 | Fbln5 | fibulin 5 | XM_006515865.3 | 86.6% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1413
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccaggaata aaaaggatac tcactgttac cattctggct ctctgtcttc 121 caagccctgg gaatgcacag gcacagtgca cgaatggctt tgacctggat cgccagtcag 181 gacagtgttt agatattgat gaatgccgaa ccatccccga ggcctgccga ggagacatga 241 tgtgtgttaa ccaaaatggc gggtatttat gcattccccg gacaaaccct gtgtatcgag 301 ggccctactc gaacccctac tcgaccccct actcaggtcc gtacccagca gctgccccac 361 cactctcagc tccaaactat cccacgatct ccaggcctct tatatgccgc tttggatacc 421 agatggatga aagcaaccaa tgtgtggatg tggacgagtg tgcaacagat tcccaccagt 481 gcaaccccac ccagatctgc atcaatactg aaggcgggta cacctgctcc tgcaccgacg 541 gatattggct tctggaaggc cagtgcttag acattgatga atgtcgctat ggttactgcc 601 agcagctctg tgcgaatgtt cctggatcct attcttgtac atgcaaccct ggttttaccc 661 tcaatgagga tggaaggtct tgccaagatg tgaacgagtg tgccaccgag aacccctgcg 721 tgcaaacctg cgtcaacacc tacggctctt tcatctgccg ctgtgaccca ggatatgaac 781 ttgaggaaga tggcgttcat tgcagtgata tggacgagtg cagcttctct gagttcctct 841 gccaacatga gtgtgtgaac cagcccggca catacttctg ctcctgccct ccaggctaca 901 tcctgctgga tgacaaccga agctgccaag acatcaacga atgtgagcac aggaaccaca 961 cgtgcaacct gcagcagacg tgctacaatt tacaaggggg cttcaaatgc atcgacccca 1021 tccgctgtga ggagccttat ctgaggatca gtgataaccg ctgtatgtgt ccTGCTGAGA 1081 ACCCTGGCTG CAGAGACCAG CCCTTTACCA TCTTGTACCG GGACATGGAC GTGGTGTCAG 1141 GACGCTCCGT TCCCGCTGAC ATCTTCCAAA TGCAAGCCAC GACCCGCTAC CCTGGGGCCT 1201 ATTACATTTT CCAGATCAAA TCTGGGAATG AGGGCAGAGA ATTTTACATG CGGCAAACGG 1261 GCCCCATCAG TGCCACCCTG GTGATGACAC GCCCCATCAA AGGGCCCCGG GAAATCCAGC 1321 TGGACTTGGA AATGATCACT GTCAACACTG TCATCAACTT CAGAGGCAGC TCCGTGATCC 1381 GACTGCGGAT ATATGTGTCG CAGTACCCAT TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1441 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1501 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 1561 CTAATCCCCG TCTACTAGGA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1621 ttgtgaaaga tt