Construct: ORF TRCN0000476716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001506.1_s317c1
- Derived from:
- ccsbBroadEn_06854
- DNA Barcode:
- AAACCAGCATGCCCAAGATACGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPH2 (5961)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5961 | PRPH2 | peripherin 2 | NM_000322.5 | 99.5% | 98.8% | (many diffs) |
| 2 | human | 5961 | PRPH2 | peripherin 2 | XR_427834.4 | 47.9% | (many diffs) | |
| 3 | human | 5961 | PRPH2 | peripherin 2 | XR_926295.3 | 18% | (many diffs) | |
| 4 | mouse | 19133 | Prph2 | peripherin 2 | NM_008938.1 | 87.4% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1107
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgctactg aaagtcaagt ttgaccagaa gaagcgggtc aagttggccc 121 aagggctctg gctcatgaac tggttctccg tgttggctgg catcatcatc ttcagcctag 181 gactgttcct gaagattgga ctccgaaaga ggagcgatgt gatgaataat tctgagagcc 241 attttgtgcc caactcattg atagggatgg gggtgctatc ctgtgtcttc aactcgctgg 301 ctgggaagat ctgctacgac gccctggacc cagccaagta tgccagatgg aagccctggc 361 tgaagccgta cctggctatc tgtgtcctct tcaacatcat cctcttcctt gtggctctct 421 gctgctttct gcttcggggc tcgctggaga acaccctggg ccaagggctc aagaacggca 481 tgaagtacta ccgggacaca gacacccctg gcaggtgttt catgaagaag accatcgaca 541 tgctgcagat cgagttcaaa tgctgcggca acaacggttt tcgggactgg tttgagattc 601 agtggatcag caatcgctac ctggactttt cctccaaaga agtcaaagat cgaatcaaga 661 gcaacgtgga tgggcggtac ctggtggacg gcgtcccttt cagctgctgc aatcctagct 721 cgccacggcc ctgcatccag tatcagaTCA CCAACAACTC AGCACACTAC AGTTACGACC 781 ACCAGACGGA GGAGCTCAAC CTGTGGGTGC GTGGCTGCAG GGCTGCCCTG CTGAGCTACT 841 ACAGCAGCCT CATGAACTCC ATGGGTGTCG TCACGCTCCT CATTTGGCTC TTCGAGGTGA 901 CCATTACAAT TGGGCTGCGC TACCTACAGA CGTCGCTGGA TGGTGTGTCC AACCCCGAGG 961 AATCTGAGAG CGAGAGCGAG GGCTGGCTGC TGGAGAAGAG CGTGCCGGAG ACCTGGAAGG 1021 CCTTTCTGGA GAGTGTGAAG AAGCTGGGCA AGGGCAACCA GGTGGAAGCC GAGGGCGCAG 1081 GCGCAGGCCA GGCCCCAGAG GCTGGCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGA 1201 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAAACCAGCA 1261 TGCCCAAGAT ACGTAACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 1321 agatt