Construct: ORF TRCN0000476741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013736.1_s317c1
- Derived from:
- ccsbBroadEn_08743
- DNA Barcode:
- AGGCGGAGGATTTCAAATCTTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TXNDC16 (57544)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57544 | TXNDC16 | thioredoxin domain containi... | NM_020784.3 | 99.8% | 99.2% | (many diffs) |
| 2 | human | 57544 | TXNDC16 | thioredoxin domain containi... | NM_001160047.1 | 99.2% | 98.6% | (many diffs) |
| 3 | human | 57544 | TXNDC16 | thioredoxin domain containi... | XR_001750464.1 | 56.2% | (many diffs) | |
| 4 | human | 57544 | TXNDC16 | thioredoxin domain containi... | XM_017021505.1 | 54.9% | 54.1% | (many diffs) |
| 5 | human | 57544 | TXNDC16 | thioredoxin domain containi... | XR_001750466.1 | 54.3% | (many diffs) | |
| 6 | human | 57544 | TXNDC16 | thioredoxin domain containi... | XR_001750465.1 | 52.8% | (many diffs) | |
| 7 | human | 57544 | TXNDC16 | thioredoxin domain containi... | XR_001750463.1 | 51.7% | (many diffs) | |
| 8 | human | 57544 | TXNDC16 | thioredoxin domain containi... | XR_001750462.1 | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2538
- ORF length:
- 2469
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttttccggc ttcaatgtct ttagagttgg gatctctttt gtcataatgt 121 gcatttttta catgccaaca gtaaactctt taccagaact gagtcctcag aaatatttta 181 gtacattgca accaggaaaa gcctctttag cttatttttg tcaagctgat tccccaagaa 241 catctgtatt tcttgaagaa ctgaatgagg ctgttagacc tctgcaggac tatggaattt 301 cagttgccaa ggttaattgt gtcaaagaag aaatatcaag atactgtgga aaagaaaagg 361 atttgatgaa agcatattta ttcaagggca acatattgct cagagaattc cctactgaca 421 ccttgtttga tgtgaatgcc attgtcgccc atgttctctt tgctcttctt tttagtgaag 481 tgaaatatat taccaacctg gaagaccttc agaacataga aaatgctctg aaaggaaaag 541 caaatattat attctcatat gtaagagcca ttggaatacc agagcacaga gcagtcatgg 601 aagccgcttt tgtgtatggg actacatacc aatttgtctt aaccacagaa attgcccttt 661 tggaaagtat tggctctgag gatgtggaat atgcacatct ctactttttt cattgtaaac 721 tagtcttgga cttgacccag caatgtagaa gaacactaat ggaacagcca ttgactacac 781 tgaacattca cctgtttatt aagacaatga aagcacctct gttgactgaa gttgctgaag 841 atcctcaaca agtttcaact gtccatctcc aactgggctt accactggtt tttattgtta 901 gccaacaggc tacttatgaa gctgatagaa gaactgcaga atgggttgct tggcgtcttc 961 tgggaaaagc aggagttcta ctcttgttaa gggactcttt ggaagtgaac attcctcaag 1021 atgctaatgt ggtcttcaaa agagcagaag agggagttcc agtggaattt ttggtattac 1081 atgatgttga tttaataata tctcatgtgg aaaataatat gcacattgag gaaatacaag 1141 aagatgaaga caatgacatg gaaggtccag atatagatgt tcaggatgat gaagtggcag 1201 aaactgtttt cagagatagg aagagaaaat tacctttgga acttacagtg gaactaacag 1261 aagaaacatt taatgcaaca gtgatggctt ctgacagcat agtactcttc tatgctggtt 1321 ggcaagcagt atccatggca tttttgcaat cctatattga tgtggcagtt aaactgaaag 1381 gcacatctac tatgcttctt actagaataa actgtgcaga ttggtctgat gtatgtacta 1441 agcaaaatgt tactgaattt cctatcataa agatgtacaa gaaaggcgag aacccagtat 1501 cttatgctgg aatgttagga accaaagatc tcctaaaatt tatccagctc aacaggattt 1561 catatccagt gaatataaca tcgatccaag aagcagaaga atatttaagt ggggaattat 1621 ataaagacct catcttgtat tctagtgtgt cagtattggg actatttagt ccaaccatga 1681 aaacagcaaa agaagatttt agtgaagcag gaaactacct aaaaggatat gttatcactg 1741 gaatttattc tgaagaagat gttttgctac tgtcaaccaa atatgctgca agtcttccag 1801 ccctgctgct tgccagacac acagaaggca aaatagagag catcccacta gctagcacac 1861 atgcacaaga catagttcaa ataataacag atgcactact ggaaatgttt ccggaaatca 1921 ctgtggaaaa tcttcccagt tatttcagac ttcagaaacc attattgatt ttgttcagtg 1981 atggcactgt aaatcctcaa tataaaaaag caatattgac actggtaaag cagaaatact 2041 tggattcatt tactccatgc tggttaaatc taaagaatac tccagtgggg agaggaatct 2101 tgcgggcata ttttgatcct ctgcctcccc ttcctcttct tgttttggtg aatctgcatt 2161 caggtggcca agtatttgca tttccttcag accaggctat aattgaagaa aaCCTTGTAT 2221 TGTGGCTGAA GAAATTAGAA GCAGGACTAG AAAATCATAT CACAATTTTA CCTGCTCAAG 2281 AATGGAAACC TCCTCTTCCA GCTTATGATT TTCTAAGTAT GATAGATGCC GCAACATCTC 2341 AACGTGGCAC TAGGAAAGTT CCCAAGTGTA TGAAAGAAAC AGATGTGCAG GAGAATGATA 2401 AGGAACAACA TGAAGATAAA TCGGCAGTCA GAAAAGAACC GATTGAAACT CTGAGAATAA 2461 AGCATTGGAA TAGAAGTAAT TGGTTTAAAG AAGCAGAAAA ATCATTTAGA CGTGATAAAG 2521 AGTTGGATGC TCAAAAGTGA ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 2581 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 2641 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG GCGGAGGATT 2701 TCAAATCTTC TCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 2761 tt