Construct: ORF TRCN0000476750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011378.1_s317c1
- Derived from:
- ccsbBroadEn_01540
- DNA Barcode:
- ATTGTGACCCCGATACGACAAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC2A1 (6513)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6513 | SLC2A1 | solute carrier family 2 mem... | NM_006516.3 | 100% | 100% | |
| 2 | mouse | 20525 | Slc2a1 | solute carrier family 2 (fa... | NM_011400.3 | 89.5% | 97.1% | (many diffs) |
| 3 | mouse | 20525 | Slc2a1 | solute carrier family 2 (fa... | XM_006502908.1 | 88.7% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1545
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcccagc agcaagaagc tgacgggtcg cctcatgctg gccgtgggag 121 gagcagtgct tggctccctg cagtttggct acaacactgg agtcatcaat gccccccaga 181 aggtgatcga ggagttctac aaccagacat gggtccaccg ctatggggag agcatcctgc 241 ccaccacgct caccacgctc tggtccctct cagtggccat cttttctgtt gggggcatga 301 ttggctcctt ctctgtgggc cttttcgtta accgctttgg ccggcggaat tcaatgctga 361 tgatgaacct gctggccttc gtgtccgccg tgctcatggg cttctcgaaa ctgggcaagt 421 cctttgagat gctgatcctg ggccgcttca tcatcggtgt gtactgcggc ctgaccacag 481 gcttcgtgcc catgtatgtg ggtgaagtgt cacccacagc ccttcgtggg gccctgggca 541 ccctgcacca gctgggcatc gtcgtcggca tcctcatcgc ccaggtgttc ggcctggact 601 ccatcatggg caacaaggac ctgtggcccc tgctgctgag catcatcttc atcccggccc 661 tgctgcagtg catcgtgctg cccttctgcc ccgagagtcc ccgcttcctg ctcatcaacc 721 gcaacgagga gaaccgggcc aagagtgtgc taaagaagct gcgcgggaca gctgacgtga 781 cccatgacct gcaggagatg aaggaagaga gtcggcagat gatgcgggag aagaaggtca 841 ccatcctgga gctgttccgc tcccccgcct accgccagcc catcctcatc gctgtggtgc 901 tgcagctgtc ccagcagctg tctggcatca acgctgtctt ctattactcc acgagcatct 961 tcgagaaggc gggggtgcag cagcctgtgt atgccaccat tggctccggt atcgtcaaca 1021 cggccttcac tgtcgtgtcg ctgtttgtgg tggagcgagc aggccggcgg accctgcacc 1081 tcataggcct cgctggcatg gcgggttgtg ccatactcat gaccatcgcg ctagcactgc 1141 tggagcagct accctggatg tcctatctga gcatcgtggc catctttggc tttgtggccT 1201 TCTTTGAAGT GGGTCCTGGC CCCATCCCAT GGTTCATCGT GGCTGAACTC TTCAGCCAGG 1261 GTCCACGTCC AGCTGCCATT GCCGTTGCAG GCTTCTCCAA CTGGACCTCA AATTTCATTG 1321 TGGGCATGTG CTTCCAGTAT GTGGAGCAAC TGTGTGGTCC CTACGTCTTC ATCATCTTCA 1381 CTGTGCTCCT GGTTCTGTTC TTCATCTTCA CCTACTTCAA AGTTCCTGAG ACTAAAGGCC 1441 GGACCTTCGA TGAGATCGCT TCCGGCTTCC GGCAGGGGGG AGCCAGCCAA AGTGACAAGA 1501 CACCCGAGGA GCTGTTCCAT CCCCTGGGGG CTGATTCCCA AGTGTTGCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA ATTGTGACCC CGATACGACA AGCGACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt