Construct: ORF TRCN0000476755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016523.1_s317c1
- Derived from:
- ccsbBroadEn_12213
- DNA Barcode:
- CAGATATTACTAAGCATGACTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEMGN (55363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476755
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55363 | HEMGN | hemogen | NM_018437.5 | 99.7% | 99.5% | 287_289delTAG |
| 2 | human | 55363 | HEMGN | hemogen | NM_197978.3 | 99.7% | 99.5% | 287_289delTAG |
| 3 | human | 55363 | HEMGN | hemogen | XM_005252086.1 | 99.7% | 99.5% | 287_289delTAG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1518
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatttggga aaggaccaat ctcatttgaa gcaccatcag acacctgacc 121 ctcatcaaga agagaaccat tctccagaag tcattggaac ctggagtttg agaaacagag 181 aactacttag aaaaagaaaa gctgaagtgc atgaaaagga aacatcacaa tggctatttg 241 gagaacagaa aaaacgcaag cagcagagaa caggaaaagg aaatcgaaga ggcagaaaga 301 gacaacaaaa cacagaattg aaggtggagc ctcagccaca gatagaaaag gaaatggaga 361 aagcactggc acctatagag aaaaaaactg agccacctgg gagcataacc aaagtatttc 421 cttcagtagc ctccccgcaa aaagttgtgc ctgaggaaca cttttctgaa atatgtcaag 481 aaagtaacat atatcaggag aatttttctg agtaccaaga aatagcagta caaaaccatt 541 cttctgaaac atgccaacat gtgtctgaac ctgaagacct ctctcctaaa atgtaccaag 601 aaatatctgt acttcaagac aattcttcca aaatatgcca agacatgaag gaacctgaag 661 acaactctcc taacacatgc caagtaatat ctgtaattca agaccatcct ttcaaaatgt 721 accaagatat ggctaaacga gaagatctgg ctcctaaaat gtgccaagaa gctgctgtac 781 ccaaaatcct tccttgtcca acatctgaag acacagctga tctggcagga tgctctcttc 841 aagcatatcc aaaaccagat gtgcctaaag gctatattct tgacacagac caaaatccag 901 cagaaccaga ggaatacaat gaaacagatc aaggaatagc tgagacagaa ggcctttttc 961 ctaaaataca agaaatagct gagcctaaag acctttctac aaaaacacac caagaatcag 1021 ctgaacctaa ataccttcct cataaaacat gtaacgaaat tattgtgcct aaagccccct 1081 ctcataaaac aatccaagaa acacctcatt ctgaagacta ttcaattgaa ataaaccaag 1141 aaactcctgg gtctgaaaaa tattcacctg aaacgtatca agaaatacct gggcttgaag 1201 aatattcacc tgaaatatac caagaaacaT CCCAGCTTGA AGAATATTCA CCTGAAATAT 1261 ACCAAGAAAC ACCGGGGCCT GAAGACCTCT CTACTGAGAC ATATAAAAAT AAGGATGTGC 1321 CTAAAGAATG CTTTCCAGAA CCACACCAAG AAACAGGTGG GCCCCAAGGC CAGGATCCTA 1381 AAGCACACCA GGAAGATGCT AAAGATGCTT ATACTTTTCC TCAAGAAATG AAAGAAAAAC 1441 CCAAAGAAGA GCCAGGAATA CCAGCAATTC TGAATGAGAG TCATCCAGAA AATGATGTCT 1501 ATAGTTATGT TTTGTTTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAGATAT TACTAAGCAT 1681 GACTTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt