Construct: ORF TRCN0000476758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002973.1_s317c1
- Derived from:
- ccsbBroadEn_00984
- DNA Barcode:
- CTTATATGGGCAACACTCGCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST6 (4166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4166 | CHST6 | carbohydrate sulfotransfera... | NM_021615.5 | 100% | 100% | |
| 2 | human | 4166 | CHST6 | carbohydrate sulfotransfera... | XM_005255955.5 | 100% | 100% | |
| 3 | human | 4166 | CHST6 | carbohydrate sulfotransfera... | XM_011523085.3 | 100% | 100% | |
| 4 | human | 23563 | CHST5 | carbohydrate sulfotransfera... | NM_024533.5 | 82.1% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1254
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggctgccg cgcgtctcca gcacagcagt gaccgcgctc ctcctggcgc 121 agaccttcct cctcctcttt ctggtttccc ggccagggcc ctcgtcccca gcaggcggcg 181 aggcgcgcgt gcatgtgctg gtgctgtcct cgtggcgctc gggctcgtcc ttcgtgggcc 241 aactcttcaa ccagcacccc gacgtcttct acctaatgga gcccgcgtgg cacgtgtgga 301 ccaccctgtc gcagggcagc gccgcaacgc tgcacatggc tgtgcgcgac ctggtgcgct 361 ccgtcttcct gtgcgacatg gacgtgtttg atgcctatct gccttggcgc cgcaacctgt 421 ccgacctctt ccagtgggcc gtgagccgtg cactgtgctc gccacccgcc tgcagtgcct 481 ttccccgagg cgccatcagc agcgaggccg tgtgcaagcc actgtgcgcg cggcagtcct 541 tcaccctggc ccgggaggcc tgccgctcct acagccacgt ggtgctcaag gaggtgcgct 601 tcttcaacct gcaggtgctc tacccgctgc tcagcgaccc cgcgctcaac ctacgcatcg 661 tgcacctggt gcgcgacccg cgggccgtgc tgcgctcccg ggagcagaca gccaaggctc 721 tggcgcgtga caacggcatc gtgctgggca ccaacggcac gtgggtggag gccgaccccg 781 gcctgcgcgt ggtgcgcgag gtgtgccgta gccacgtacg catcgccgag gccgccacac 841 tcaagccgcc accctttctg cgcggccgct accgccTGGT GCGCTTCGAG GACCTGGCGC 901 GGGAGCCGCT GGCAGAAATC CGTGCGCTCT ACGCCTTCAC TGGGCTCAGT CTCACGCCAC 961 AGCTCGAGGC CTGGATCCAT AACATCACCC ACGGATCTGG ACCTGGTGCG CGCCGCGAAG 1021 CCTTCAAGAC TTCGTCCAGG AATGCGCTCA ACGTCTCCCA GGCCTGGCGC CATGCGCTGC 1081 CCTTTGCCAA GATCCGCCGC GTGCAGGAAC TGTGCGCTGG TGCGCTGCAG CTGCTGGGCT 1141 ACCGGCCTGT GTACTCTGAG GACGAGCAGC GCAACCTCGC CCTTGATCTG GTGCTGCCAC 1201 GAGGCCTGAA CGGCTTCACT TGGGCATCAT CCACCGCCTC GCACCCCCGA AATTTGCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAC TTATATGGGC AACACTCGCC GTCACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att