Construct: ORF TRCN0000476766
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002947.1_s317c1
- Derived from:
- ccsbBroadEn_02395
- DNA Barcode:
- TATGCATACGAGCTGTTCTTACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS26C (10311)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476766
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10311 | VPS26C | VPS26 endosomal protein sor... | NM_006052.2 | 100% | 100% | |
| 2 | human | 10311 | VPS26C | VPS26 endosomal protein sor... | NM_001331022.1 | 90.9% | 90.9% | 351_352ins81 |
| 3 | human | 10311 | VPS26C | VPS26 endosomal protein sor... | NM_001331021.1 | 83.8% | 83.8% | 56_57ins144 |
| 4 | human | 10311 | VPS26C | VPS26 endosomal protein sor... | NM_001331018.1 | 57.9% | 57.9% | 57_58ins375 |
| 5 | mouse | 13185 | Dscr3 | DSCR3 arrestin fold containing | NM_007834.3 | 85.1% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 960
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggaccgcc ctggacatca agattaaaag agcgaataaa gtttatcacg 121 ccggggaagt gctctctggc gtggtggtca tatcgagtaa ggattcagtc caacaccagg 181 gagtgtcttt gaccatggaa ggaactgtaa acctccagct cagtgccaaa agtgtgggtg 241 tgtttgaagc tttttataat tctgttaagc ctatccagat tatcaacagc accatagaaa 301 tggtgaagcc ggggaaattt cccagcggca aaacagaaat cccttttgaa tttcctctgc 361 acttgaaggg taacaaagtt ctgtatgaga cgtatcatgg cgtgtttgtc aacattcagt 421 atacactgcg ctgtgacatg aagcggtctc tgttggccaa ggacttgaca aagacctgtg 481 aatttatcgt tcactccgct cctcagaagg ggaagtttac tcccagtccc gtggacttca 541 cgattacacc tgaaacctta cagaacgtca aagagagagc tttgcttccc aaatttctcc 601 TTCGAGGACA TCTCAACTCA ACAAACTGTG TCATCACGCA GCCACTAACG GGAGAGCTGG 661 TGGTGGAGAG CTCGGAAGCC GCCATCAGAA GCGTGGAGCT GCAGCTGGTG CGCGTGGAGA 721 CGTGCGGGTG TGCAGAAGGC TATGCCCGCG ACGCCACGGA GATTCAGAAC ATTCAGATCG 781 CCGACGGGGA TGTGTGCAGG GGCCTCTCTG TCCCCATCTA CATGGTCTTC CCTAGGCTGT 841 TCACCTGCCC TACACTGGAG ACCACCAACT TCAAAGTGGA ATTTGAGGTT AACATCGTGG 901 TGCTGCTTCA CCCTGACCAC CTCATCACGG AGAACTTCCC GCTGAAGCTC TGCAGGATAT 961 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGATATGC ATACGAGCTG TTCTTACCGA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt