Construct: ORF TRCN0000476769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015724.1_s317c1
- Derived from:
- ccsbBroadEn_01245
- DNA Barcode:
- ACTACTACAACTCTGCGTTAACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POMC (5443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5443 | POMC | proopiomelanocortin | NM_000939.4 | 100% | 100% | |
| 2 | human | 5443 | POMC | proopiomelanocortin | NM_001035256.2 | 100% | 100% | |
| 3 | human | 5443 | POMC | proopiomelanocortin | NM_001319204.2 | 100% | 100% | |
| 4 | human | 5443 | POMC | proopiomelanocortin | NM_001319205.2 | 100% | 100% | |
| 5 | mouse | 18976 | Pomc | pro-opiomelanocortin-alpha | NM_001278581.1 | 75.7% | 71.9% | (many diffs) |
| 6 | mouse | 18976 | Pomc | pro-opiomelanocortin-alpha | NM_001278582.1 | 75.7% | 71.9% | (many diffs) |
| 7 | mouse | 18976 | Pomc | pro-opiomelanocortin-alpha | NM_001278583.1 | 75.7% | 71.9% | (many diffs) |
| 8 | mouse | 18976 | Pomc | pro-opiomelanocortin-alpha | NM_001278584.1 | 75.7% | 71.9% | (many diffs) |
| 9 | mouse | 18976 | Pomc | pro-opiomelanocortin-alpha | NM_008895.4 | 75.7% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 870
- ORF length:
- 801
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgagatcg tgctgcagcc gctcgggggc cctgttgctg gccttgctgc 121 ttcaggcctc catggaagtg cgtggctggt gcctggagag cagccagtgt caggacctca 181 ccacggaaag caacctgctg gagtgcatcc gggcctgcaa gcccgacctc tcggccgaga 241 ctcccatgtt cccgggaaat ggcgacgagc agcctctgac cgagaacccc cggaagtacg 301 tcatgggcca cttccgctgg gaccgattcg gccgccgcaa cagcagcagc agcggcagca 361 gcggcgcagg gcagaagcgc gaggacgtct cagcgggcga agactgcggc ccgctgcctg 421 agggcggccc cgagccccgc agcgatggtg ccaagccggg cccgcgcgag ggcaagcgct 481 ccTACTCCAT GGAGCACTTC CGCTGGGGCA AGCCGGTGGG CAAGAAGCGG CGCCCAGTGA 541 AGGTGTACCC TAACGGCGCC GAGGACGAGT CGGCCGAGGC CTTCCCCCTG GAGTTCAAGA 601 GGGAGCTGAC TGGCCAGCGA CTCCGGGAGG GAGATGGCCC CGACGGCCCT GCCGATGACG 661 GCGCAGGGGC CCAGGCCGAC CTGGAGCACA GCCTGCTGGT GGCGGCCGAG AAGAAGGACG 721 AGGGCCCCTA CAGGATGGAG CACTTCCGCT GGGGCAGCCC GCCCAAGGAC AAGCGCTACG 781 GCGGTTTCAT GACCTCCGAG AAGAGCCAGA CGCCCCTGGT GACGCTGTTC AAAAACGCCA 841 TCATCAAGAA CGCCTACAAG AAGGGCGAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 901 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 961 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACTAC 1021 TACAACTCTG CGTTAACTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1081 tgaaagatt