Construct: ORF TRCN0000476772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012554.1_s317c1
- Derived from:
- ccsbBroadEn_06621
- DNA Barcode:
- CTCAGAGCATCCGCGATCTTCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA8 (4702)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476772
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4702 | NDUFA8 | NADH:ubiquinone oxidoreduct... | NM_014222.3 | 99.6% | 100% | 126G>A;219G>A |
| 2 | human | 4702 | NDUFA8 | NADH:ubiquinone oxidoreduct... | NM_001318195.2 | 73.6% | 73.8% | 126G>A;219G>A;383_384delGGins134 |
| 3 | mouse | 68375 | Ndufa8 | NADH dehydrogenase (ubiquin... | NM_026703.2 | 83.2% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggggatagtg gagctgccca ctctagagga gctgaaagta gatgaggtga 121 aaattagttc tgctgtgctt aaagctgcgg cccatcacta tggagctcaa tgtgataagc 181 ccaacaagga atttatgctc tgccgctggg aagagaaaga tccgaggcgg tgtttaGAGG 241 AAGGCAAACT GGTCAACAAG TGTGCTTTGG ACTTCTTTAG GCAAATAAAA CGTCACTGTG 301 CAGAGCCTTT TACAGAATAT TGGACTTGCA TTGATTATAC TGGCCAGCAG TTATTTCGTC 361 ACTGTCGCAA ACAGCAGGCA AAGTTTGACG AGTGTGTGCT GGACAAACTG GGCTGGGTGC 421 GGCCTGACCT GGGAGAACTG TCAAAGGTCA CCAAAGTGAA AACAGATCGA CCTTTACCGG 481 AGAATCCCTA TCACTCAAGA CCAAGACCGG ATCCCAGCCC TGAGATCGAG GGAGATCTGC 541 AGCCTGCCAC ACATGGCAGC CGCTTTTATT TCTGGACCAA GTACCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGACTC AGAGCATCCG CGATCTTCGT CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t